Cloning and structural characterization of juvenile hormone diol kinase in Spodoptera litura
Cloning and structural characterization of juvenile hormone diol kinase in Spodoptera litura
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DOI：
10.1111/1744-7917.12236
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发表时间：
2016-12
期刊：
影响因子：
4
通讯作者：
Baojuan Zeng;Yuan Lv;Li-li Zhang;Li-Hua Huang;Q. Feng
中科院分区：
文献类型：
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作者：
Baojuan Zeng;Yuan Lv;Li-li Zhang;Li-Hua Huang;Q. Feng
保幼激素（JH）是昆虫变态过程中的重要激素之一。保幼激素二醇激酶（JHDK）是一种参与JH代谢的酶，催化JH二醇形成无JH活性的极性终产物JH二醇磷酸。本研究从斜纹夜蛾中克隆了一个JHDK互补DNA（cDNA），并对该基因的结构和表达进行了研究。该cDNA全长714bp，编码183个氨基酸，分子量21kDa，等电点4.55。基于结构，预测蛋白质中有三个推定的钙结合基序和三磷酸鸟苷结合基序。三维结构的建模表明，该蛋白质由8个α螺旋与环连接组成，没有β折叠。该基因在5龄和6龄幼虫的表皮、脂肪体和中肠中均有表达。表皮中的表达水平低于脂肪体和中肠。该基因在五龄、六龄中肠和脂肪体发育的早期表达量高于后期。结果表明，该基因可能参与了血吸虫幼虫JH滴度的调控。斜纹。
Juvenile hormone (JH) is one of the key insect hormones that regulate metamorphosis. Juvenile hormone diol kinase (JHDK) is an enzyme involved in JH metabolism and catalyzes JH diol to form a polar end product, JH diol phosphate that has no JH activity. In this study, a JHDK complementary DNA (cDNA) was cloned from Spodoptera litura and the structure and expression of the gene was characterized. The cDNA was 714 base pairs in length and encoded a protein of 183 amino acids with a molecular mass of 21 kDa and an isoelectric point of 4.55. Based on the structure, three putative calcium binding motifs and guanosine triphosphate‐binding motifs were predicted in the protein. Modeling of the 3‐D structure showed that the protein consisted of eight α‐helixes linked with loops, with no β‐sheets. The gene was expressed in the epidermis, fat body and midgut of fifth and sixth instar larvae. The expression level in the epidermis was lower than in the fat body and midgut. The gene was expressed at higher levels at the early stages than in the later stages of fifth and sixth instar midgut and fat body. The results suggest that this gene may be involved in the regulation of the JH titer in larvae of S. litura.