Xenopus as an experimental model for studying evolution of hsp-immune system interactions
Xenopus as an experimental model for studying evolution of hsp-immune system interactions
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DOI：
10.1016/s1046-2023(03)00186-5
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发表时间：
2004-01-01
期刊：
影响因子：
4.8
通讯作者：
Maniero, GD
中科院分区：
文献类型：
--
作者：
Robert, J;Gantress, J;Maniero, GD
蛙爪蟾为研究热休克蛋白免疫学特性的进化保守性提供了一个独特的模型系统。维持和免疫同基因克隆的定义的MHC基因型的一般方法一起提出了最近开发的协议，探索唇介导的免疫反应在体外（增殖和细胞毒性试验）和体内（过继细胞转移和抗体治疗）在成人和自然MHC I类缺陷的幼虫。最后，技术研究内质网驻留的gp96在肿瘤和正常淋巴细胞的细胞表面的表达方式被认为是。(C)2003年爱思唯尔公司All rights reserved.
The frog Xenopus provides a unique model system for studying the evolutionary conservation of the immunological properties of heat shock proteins (hsps). General methods for maintaining and immunizing isogenetic clones of defined MHC genotypes are presented together with more recently developed protocols for exploring lisp-mediated immune responses in vitro (proliferative and cytotoxic assays) and in vivo (adoptive cell transfer and antibody treatment) in adults and in naturally MHC class I-deficient larvae. Finally, techniques to study modalities of expression of the endoplasmic reticulum resident gp96 at the cell surface of tumor and normal lymphocytes are considered. (C) 2003 Elsevier Inc. All rights reserved.