Autophagy eliminates cytoplasmic β-catenin and NICD to promote the cardiac differentiation of P19CL6 cells
Autophagy eliminates cytoplasmic β-catenin and NICD to promote the cardiac differentiation of P19CL6 cells
复制标题
自噬消除细胞质β-catenin和NICD促进P19CL6细胞的心脏分化
DOI：
10.1016/j.cellsig.2014.07.028
复制
发表时间：
2014-11-01
影响因子：
4.8
通讯作者：
Zhou, Chunyan
中科院分区：
文献类型：
--
作者：
Jia, Zhuqing;Wang, Jiaji;Zhou, Chunyan
Autophagy plays important roles in adipogenesis and neuron development. However, how autophagy contributes to cardiac development is not well understood. The main aim of our study was to determine the association between autophagy and myocardial differentiation and its roles in this process. Using a well-established in vitro cardiomyocyte differentiation system, P19CL6 cells, we found that autophagy occurred from the early stage of cardiac differentiation. Blocking autophagy by knocking-down of autophagy-related gene Atg7 or Atg5 inhibited the cardiac differentiation of P19CL6 cells. Further investigation demonstrated that LO and P62 could form a complex with S-catenin and NICD, respectively, and promoted the degradation of beta-catenin and NICD. Enhancing autophagy promoted the formation of complex, whereas blocking autophagy attenuated the degradation of beta-catenin and NICD. Taken together, autophagy could facilitate P19CL6 cells to complete the cardiac differentiation process through blocking Wnt and Notch signaling pathways. (C) 2014 The Authors. Published by Elsevier Inc.
