Effect of Calcium Concentration in Growth Medium on Oxalate Content and Evaluation of the Role of Guttation in the Regulation of Oxalate in Eddo
Effect of Calcium Concentration in Growth Medium on Oxalate Content and Evaluation of the Role of Guttation in the Regulation of Oxalate in Eddo
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DOI：
10.1626/pps.18.464
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发表时间：
2015-01
影响因子：
2.5
通讯作者：
M. Islam;Hayato Maeda;M. Kawasaki
中科院分区：
文献类型：
--
作者：
M. Islam;Hayato Maeda;M. Kawasaki
以香芋（Colocasia esculenta（L.）肖特湾antiquorum Hubbard & Schmidt）.植物在含有0 mM钙、1 mM硝酸钙（对照）、15 mM硝酸钙或15 mM氯化钙的溶液中水培生长。叶片、叶柄和球茎的总草酸含量（可溶性加不溶性）与含有1 mM或15 mM硝酸钙或0 mM钙的溶液中的钙浓度没有差异。这些部位的可溶性草酸盐含量随着溶液中钙浓度的增加而降低。含有15 mM硝酸钙或15 mM氯化钙的溶液中，可溶性草酸盐含量与总草酸盐含量的比例显着降低，尤其是在叶片中，比0 mM钙或1 mM硝酸钙。与此相反，不溶性草酸含量和钙浓度之间呈正相关关系。这些结果表明，生长培养基中的高钙浓度降低了植物的可溶性草酸盐含量。Eddo滴液中检测到可溶性草酸盐。在含有15 mM钙的溶液中，该液体中的可溶性草酸盐含量（mg mL-1）和通过点滴渗出的可溶性草酸盐的量（mg叶-1夜-1）显著低于含有0 mM和1 mM钙的溶液。这些结果表明，滴注可能会影响植物体中的可溶性草酸盐的浓度，虽然没有强烈的贡献，在高钙条件下生长的埃多可溶性草酸盐含量的减少。
The effects of calcium concentrations in the growth medium on oxalate content of leaf blades, petioles and corms and the involvement of guttation in the regulation of oxalate homeostasis were investigated in eddo (Colocasia esculenta (L.) Schott var. antiquorum Hubbard & Rehder). The plants were grown hydroponically in solutions containing 0 mM calcium, 1 mM calcium nitrate (control), 15 mM calcium nitrate or 15 mM calcium chloride. Total oxalate content (soluble plus insoluble) of leaf blades, petioles and corms did not differ with the calcium concentration in solutions containing 1 mM or 15 mM calcium nitrate or 0 mM calcium. The soluble oxalate content of these parts decreased as the calcium concentration of the solution was increased. Solutions containing 15 mM calcium nitrate or 15 mM calcium chloride gave a significantly lower proportion of soluble oxalate content to total oxalate content in each part, especially in leaf blades than 0 mM calcium or 1 mM calcium nitrate. In contrast, a positive correlation was found between insoluble oxalate content and calcium concentration in the solution. These results demonstrate that high calcium concentrations in the growth medium reduce soluble oxalate content of the plant. Soluble oxalate was detected in eddo guttation fluid. Soluble oxalate content in this fluid (mg mL−1) and the amount of soluble oxalate exuded by guttation (mg leaf−1 night−1) were significantly lower in the solutions containing 15 mM calcium than in those containing 0 mM and 1 mM calcium. These results indicate that guttation may affect the concentration of soluble oxalate in the plant bodies although not strongly contributing to a decrease in soluble oxalate content in eddo grown under high calcium conditions.