Expression profile of EFNB1, EFNB2, two ligands of EPHB2 in human gastric cancer
Expression profile of EFNB1, EFNB2, two ligands of EPHB2 in human gastric cancer
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DOI：
10.1007/s00432-002-0355-0
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发表时间：
2002-07-01
影响因子：
3.6
通讯作者：
Sugimura, H
中科院分区：
文献类型：
--
作者：
Kataoka, H;Tanaka, M;Sugimura, H
目的：我们以前曾报道过EPHB 2在胃癌中过表达，然而，其配体EFNB 1和EFNB 2的表达谱尚不清楚。本研究旨在探讨EPHB 2及其配体EFNB 1和EFNB 2在人胃癌中的表达。研究方法：应用~（32）P半定量RT-PCR技术对29例胃癌患者的胃癌组织及相应的正常组织进行检测。结果如下：EPHB 2在胃癌组织中的表达高于正常组织（P = 0.01），21/29（72.4%）（P = 0.037）和14/29（48.3%）的患者中EFNB 1和EFNB 2分别高表达。高分化腺癌和低分化腺癌中EPHB 2的过表达频繁[分别为10113（76.9%）和9/14（64.3%）]。另一方面，EFNB 1的过度表达在低分化腺癌中比在高分化腺癌中更常见[分别为12/14（85.7%）和7/13（53.8%）]。P = 0.027]。EPHB 2和EFNB 1水平升高在早期胃癌的大量亚群中检测到。胃癌标本中这三个基因的基因组改变要么没有发现，要么很少发现，除了几个罕见的EPHB 2变异。结论：提示EPHB 2及其配体EFNB 1的表达可能与胃癌的发生有关。
Purpose: We have previously reported that EPHB2 is overexpressed in gastric cancer; however, the expression profiles of its ligands, EFNB1 and EFNB2,are unknown. This study was designed to investigate the expression of EPHB2 and its ligands, EFNB1 and EFNB2, in human gastric cancer. Methods: Semi-quantitative RT-PCR using (32)P was performed on human gastric cancer tissues and corresponding normal tissues (29 gastric cancer patients). Results: EPHB2 was more highly expressed in tumor tissues than in normal tissues in 21 out of 29 (72.4%), in gastric cancer patients (P = 0.01); EFNB1 and EFNB2 were highly expressed in 21 out of 29 (72.4%) (P = 0.037) and 14 out of 29 (48.3%) patients, respectively. The overexpression of EPHB2 was frequently detected in both well-differentiated adenocarcinoma and poorly differentiated adenocarcinoma [10113 (76.9%) and 9/14 (64.3%), respectively]. On the other hand, the overexpression of EFNB1 was more frequently detected in poorly differentiated adenocarcinoma than in well-differentiated adenocarcinoma [12/14 (85.7%) and 7/13 (53.8%), respectively. P = 0.027]. Elevated levels of EPHB2 and EFNB1 were detected in substantial subsets of early gastric cancers. Genomic alterations to these three genes in gastric cancer specimens were either not found or rarely found except for several rare variations of EPHB2. Conclusions: These findings suggest that not only the expression of EPHB2, but the expression of its ligand EFNB1 may have some relation with the oncogenesis of gastric cancer.