Monolithic Silicon Chip for Immunofluorescence Detection on Single Magnetic Beads
Monolithic Silicon Chip for Immunofluorescence Detection on Single Magnetic Beads
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DOI：
10.1021/ac902241j
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发表时间：
2010-01-01
影响因子：
7.4
通讯作者：
Gijs, Martin A. M.
中科院分区：
文献类型：
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作者：
Dupont, Emile P.;Labonne, Estelle;Gijs, Martin A. M.
虽然荧光检测由于其高灵敏度而广泛用于生物测定，但由光源、滤光器、显微镜主体和相机组成的完整荧光显微镜设置仍然是笨重的设备，从而损害了其在护理点环境中的使用。在这里，我们提出了一个集成的单片硅芯片集成磁操纵和荧光标记的磁珠的光学检测。我们的方法允许一个单一的微粒的荧光显微镜测量。我们通过在磷酸盐缓冲盐水-牛血清白蛋白（PBS-BSA）溶液中，在磁珠表面进行的非竞争性夹心免疫测定中检测癌症生物标志物5D 10单克隆抗体（mAb），证明了这种方法的可行性，检测限为1 ng mL（-1）。
While fluorescence detection is widely used for bioassays owing to its high sensitivity, a complete fluorescent microscopy setup, comprised of a light source, optical filters, a microscope body, and a camera, still is bulky equipment, compromising its use in a point-of-care environment. Here we propose an integrated monolithic silicon chip for integrated magnetic manipulation and optical detection of fluorescently labeled magnetic beads. Our approach permits microscopeless measurement of the fluorescence of a single microparticle. We demonstrate the viability of this approach by the detection of cancer biomarker 5D10 monoclonal antibodies (mAbs) in a noncompetitive sandwich immunoassay performed on the surface of magnetic beads, in a phosphate buffered saline-bovine serum albumin (PBS-BSA) solution,,with a detection limit of 1 ng mL(-1).