Effects of Inhibitors on the Transcriptional Profiling of Gluconobater oxydans NL71 Genes after Biooxidation of Xylose into Xylonate.
Effects of Inhibitors on the Transcriptional Profiling of Gluconobater oxydans NL71 Genes after Biooxidation of Xylose into Xylonate.
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木糖生物氧化成木糖酸后抑制剂对氧化葡糖酸杆菌NL71基因转录谱的影响
DOI：
10.3389/fmicb.2017.00716
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发表时间：
2017
影响因子：
5.2
通讯作者：
Xu Y
中科院分区：
文献类型：
--
作者：
Miao Y;Shen Y;Xu Y
d -木糖酸属于30种生物质平台化学物质，代表了木糖的一个有前途的应用。直到今天，氧化葡萄糖杆菌NL71是能够将木糖发酵成木酸盐的最有效的微生物。然而，当使用浓缩的木质纤维素水解物作为底物时，由于在生物质预处理过程中形成的各种降解化合物的存在，其生长受到严重抑制。因此确定了三种关键的木质纤维素抑制剂，即甲酸、糠醛和4-羟基苯甲醛。由于微生物发酵主要是在基因组水平上调控的，因此通过对对照样品和分别用上述抑制剂处理的样品进行RNA测序，获得了四组细胞转录组进行比较研究。通过对4个转录组的比较，获得了572、714个基因和408个deg的数字基因表达谱。3种抑制剂诱导了多个与不同功能群相关的基因表现出特征性的表达模式，其中19个基因通过qRT-PCR进一步检测并确认。我们推断出许多差异表达的基因可以解释细胞对抑制效应的反应。我们提供的结果使科学界能够更好地定义木糖细胞生物氧化成木糖酸过程中微生物对木质纤维素抑制剂反应的分子过程。
D-Xylonic acid belongs to the top 30 biomass-based platform chemicals and represents a promising application of xylose. Until today, Gluconobacter oxydans NL71 is the most efficient microbe capable of fermenting xylose into xylonate. However, its growth is seriously inhibited when concentrated lignocellulosic hydrolysates are used as substrates due to the presence of various degraded compounds formed during biomass pretreatment. Three critical lignocellulosic inhibitors were thereby identified, i.e., formic acid, furfural, and 4-hydroxybenzaldehyde. As microbe fermentation is mostly regulated at the genome level, four groups of cell transcriptomes were obtained for a comparative investigation by RNA sequencing of a control sample with samples treated separately with the above-mentioned inhibitors. The digital gene expression profiles screened 572, 714 genes, and 408 DEGs was obtained by the comparisons among four transcriptomes. A number of genes related to the different functional groups showed characteristic expression patterns induced by three inhibitors, in which 19 genes were further tested and confirmed by qRT-PCR. We extrapolated many differentially expressed genes that could explain the cellular responses to the inhibitory effects. We provide results that enable the scientific community to better define the molecular processes involved in the microbes' responses to lignocellulosic inhibitors during the cellular biooxidation of xylose into xylonic acid.