Convergent evolution of complex regulatory landscapes and pleiotropy at Hox loci
Convergent evolution of complex regulatory landscapes and pleiotropy at Hox loci
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DOI：
10.1126/science.1257493
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发表时间：
2014-11-21
期刊：
影响因子：
56.9
通讯作者：
Duboule, Denis
中科院分区：
文献类型：
--
作者：
Lonfat, Nicolas;Montavon, Thomas;Duboule, Denis
在脊椎动物的手指和外生殖器的形态发生过程中，HOX基因都是必需的。我们调查了在这种不同的背景下转录是否涉及一个共享的含有增强子的环境。我们表明，相同的调控拓扑被使用，但与一些组织特异性的增强子-启动子相互作用，表明调控骨干从一个背景劫持到另一个。此外，在HoxA和HoxD簇中都观察到了类似的组织，这两个簇在祖先无脊椎动物中通过基因组复制而分离。我们认为，这种趋同的调控进化是由某些染色质结构的预先存在触发的，从而促进了随后适当转录因子的招募。这样的调控拓扑可能既有利于也制约了脊椎动物多效性发育基因的进化。
Hox genes are required during the morphogenesis of both vertebrate digits and external genitals. We investigated whether transcription in such distinct contexts involves a shared enhancer-containing landscape. We show that the same regulatory topology is used, yet with some tissue-specific enhancer-promoter interactions, suggesting the hijacking of a regulatory backbone from one context to the other. In addition, comparable organizations are observed at both HoxA and HoxD clusters, which separated through genome duplication in an ancestral invertebrate animal. We propose that this convergent regulatory evolution was triggered by the preexistence of some chromatin architecture, thus facilitating the subsequent recruitment of the appropriate transcription factors. Such regulatory topologies may have both favored and constrained the evolution of pleiotropic developmental loci in vertebrates.