Defining the genetic blueprint of kidney development.
Defining the genetic blueprint of kidney development.
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定义肾脏发育的遗传蓝图。
DOI：
10.1007/s00467-011-1807-z
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Patterson,LarryT
中科院分区：
文献类型：
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作者：
Potter,SSteven;Brunskill,EricW;Patterson,LarryT
成千上万的基因在肾脏发育过程中表现出差异表达模式，这表明驱动这一过程的遗传程序是复杂的。虽然在确定肾发生的遗传基础方面已经取得了很大进展，但很明显还有很多东西需要学习。一个全球地图集的基因表达谱的多个元素的发展中的肾脏将允许识别新的生长因子受体相互作用，确定不同的组件的额外的分子标记物，促进产生特定的GFP-CRE转基因小鼠工具的隔室，借给洞察到基因调控电路肾单位的形成，并充分表征波的基因表达，促使肾发生。微阵列和cDNA文库的下一代深度测序均可用于定义全面、灵敏和定量的基因表达谱。此外，激光捕获显微切割和转基因GFP小鼠可用于从发育中的肾脏分离特定的隔室和纯细胞类型。先进的技术甚至允许单细胞的强大基因表达谱。最终的目标是制作一个精美详细的驱动肾脏发育的基因表达程序图谱。
Thousands of genes show differential expression patterns during kidney development, suggesting that the genetic program driving this process is complex. While great progress has been made in defining the outline of the genetic basis of nephrogenesis, it is clear that much remains to be learned. A global atlas of the gene expression profiles of the multiple elements of the developing kidney would allow the identification of novel growth factor–receptor interactions, identify additional molecular markers of distinct components, facilitate the generation of compartment specific GFP-CRE transgenic mouse tools, lend insights into the genetic regulatory circuits governing nephron formation, and fully characterize the waves of gene expression that impel nephrogenesis. Both microarrays and next generation deep sequencing of cDNA libraries can be used to define comprehensive, sensitive, and quantitative gene expression profiles. In addition, laser capture microdissection and transgenic GFP mice can be used to isolate specific compartments and pure cell types from the developing kidney. Advancing technologies are even allowing robust gene expression profiling of single cells. The final goal is the production of an exquisitely detailed atlas of the gene expression program that drives kidney development.