Alterations in erythrocyte membrane protein composition in advanced non-small cell lung cancer
Alterations in erythrocyte membrane protein composition in advanced non-small cell lung cancer
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DOI：
10.1016/j.bcmd.2006.02.002
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发表时间：
2006-05-01
影响因子：
2.3
通讯作者：
Sanchez-Yague, Jesus
中科院分区：
文献类型：
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作者：
Hernandez-Hernandez, Angel;Rodriguez, Marina C.;Sanchez-Yague, Jesus
癌症可能与与红细胞（R-BC）功能相关的血液学并发症有关，其生理作用现在已经扩大，因为现在知道RBC也是信号细胞。本研究的目的是探讨晚期非小细胞肺癌（NSCLC）中红细胞蛋白组成的改变。21例晚期（III-IV期）非小细胞肺癌患者（16例鳞状细胞癌和5例腺癌）和21名健康志愿者的血液样本被使用。采用差示扫描量热法（DSC）筛选6例随机抽取的患者和6例对照者的红细胞鬼影改变。随机选择15例患者和15例对照，与DSC测量中使用的样品不同，通过SDS-PAGE和Western blotting或凝集素酶免疫测定分析糖蛋白（GP）种，带3和糖蛋白的表达。此外，5例慢性阻塞性肺疾病（COPD）患者作为良性炎性疾病的对照组。采用慢性阻塞性肺病患者的血液样本分析gp、带3和syalglyprotein的表达。我们在NSCLC中观察到以下变化：(a) GP表达水平的变化，主要在GPA和GPC单体以及GPAB二聚体中降低；(b)带3蛋白水平降低，(c)不同唾液糖蛋白表达改变。COPD患者的红细胞也显示蛋白质异常，其中一些蛋白质异常，特别是在3带和sysylocoprotein水平，与NSCLC相似。(c) 2006爱思唯尔公司版权所有。
Cancer can be associated with hematological complications related to red blood cell (R-BC) function, whose physiological roles have now been expanded since it is now known that RBC are also signalling cells. The aim of this study was to explore the alterations occurring in the protein composition of RBC in advanced non-small cell lung cancer (NSCLC). Blood samples from 21 patients with advanced (stages III-IV) NSCLC (16 squamous cell carcinomas and 5 adenocarcinomas), and from 21 healthy volunteers were used. Samples from 6 randomly selected patients and 6 controls were used for the screening of erythrocyte ghost alterations by Differential Scanning Calorimetry (DSC). Samples from 15 patients and 15 controls, different from those used in the DSC measurements, were randomly selected for analysis of the expression of glycophorin (GP) species, band 3, and glycoproteins by SDS-PAGE and Western blotting or lectin enzyme immunoassays. Additionally, 5 patients with chronic obstructive pulmonary disease (COPD) were used as a control group representative of a benign inflammatory disease. Blood samples from the COPD patients were used to analyze the expression of GPs, band 3 and syaloglycoproteins. We observed the following in NSCLC: (a) changes in GP expression levels, mainly decreases in the GPA and GPC monomers, and in the GPAB dimers; (b) a decrease in the band 3 protein level, and (c) alterations in the expression of different sialoglycoproteins. RBC from the COPD patients also showed protein abnormalities, some of them, especially at the level of band 3 and the syaloglycoproteins, being similar to those in NSCLC. (c) 2006 Elsevier Inc. All rights reserved.