Cutting Edge: Chromatin Accessibility Programs CD8 T Cell Memory.
Cutting Edge: Chromatin Accessibility Programs CD8 T Cell Memory.
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DOI：
10.4049/jimmunol.1602086
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发表时间：
2017-03-15
期刊：
影响因子：
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通讯作者：
Boss JM
中科院分区：
文献类型：
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作者：
Scharer CD;Bally AP;Gandham B;Boss JM
CD8 T cell memory is characterized by rapid recall of effector function, increased proliferation, and reduced activation requirements. Despite the extensive functional characterization, the molecular mechanisms that facilitate these enhanced properties are not well characterized. Here, ATAC-seq was employed to map the cis-regulatory elements in CD8 T cells responding to acute and chronic lymphocytic choriomeningitis virus infections. Integration of chromatin accessibility profiles with gene expression data identified unique regulatory modules that were enriched for distinct combinations of transcription factor binding motifs. Memory CD8 T cells displayed a chromatin accessibility structure that was absent from other acute and exhausted cells types and included key effector and proliferative genes. Stimulation of memory cells revealed enhanced transcription of “memory-primed” genes compared to naïve cells. Thus, memory CD8 T cells display a pre-programmed chromatin accessibility profile and maintain a molecular history of cis-element usage, thereby reducing the steps necessary to revive effector functions.
