Canine prostate carcinomas express markers of urothelial and prostatic differentiation
Canine prostate carcinomas express markers of urothelial and prostatic differentiation
复制标题
DOI：
10.1354/vp.41-2-131
复制
发表时间：
2004-03-01
影响因子：
2.4
通讯作者：
Rosol, TJ
中科院分区：
文献类型：
--
作者：
LeRoy, BE;Nadella, MVP;Rosol, TJ
前列腺癌和移行细胞癌（TCC）发生在老年犬的前列腺，在光镜下评估时具有形态学上的相似性。狗是研究人类前列腺癌的常用动物模型；因此，准确区分犬前列腺癌与tcc具有重要意义。我们研究角蛋白7 （K7）和精氨酸酯酶（AE）是否有助于犬前列腺癌与TCC的分化。应用免疫组织化学方法检测K7在正常和肿瘤犬前列腺和膀胱组织中的表达。采用northern blots和逆转录聚合酶链反应（RT-PCR）检测AE信使核糖核酸（mRNA）在正常和肿瘤犬前列腺和膀胱中的表达。此外，测定了正常和肿瘤犬前列腺和膀胱组织中AE酶的活性。我们发现K7在前列腺癌和tcc中的表达有明显的相似性。AE mRNA在正常前列腺组织中高水平表达，而在前列腺癌组织中低水平表达。巢式RT-PCR在tcc（15例中的13例）和前列腺癌（13例中的13例）中检测到AE mRNA。在正常前列腺和某些前列腺癌中，AE的酶活性较高，而正常膀胱和tcc则产生较低水平的AE。综上所述，K7和AE不能作为犬TCC与前列腺癌的鉴别指标。
Prostate carcinoma and transitional cell carcinoma (TCC) occur in the prostate gland of older dogs and have morphologic similarities when evaluated by light microscopy. The dog is a commonly used animal model for studying human prostate carcinoma; therefore, it is important to accurately differentiate canine prostate carcinomas from TCCs. We investigated whether keratin 7 (K7) and arginine esterase (AE) would aid differentiation of canine prostate carcinoma from TCC. K7 expression was evaluated in normal and neoplastic canine prostate and bladder tissues using immunohistochemistry. The expression of AE messenger ribonucleic acid (mRNA) in normal and neoplastic canine prostate and bladder was detected using northern blots and reverse transcriptase-polymerase chain reaction (RT-PCR). In addition, AE enzyme activity was measured in normal and neoplastic canine prostate and bladder tissues. We found marked similarities in K7 expression in prostate carcinomas and TCCs. AE mRNA was present in high levels in normal prostatic tissue but was reduced in prostate carcinoma by northern blot assay. Nested RT-PCR detected AE mRNA both in TCCs (13 of 15) and in prostate carcinomas (13 of 13). Enzymatic activity of AE was high in normal prostate gland and in some prostate carcinomas, whereas normal bladder and TCCs produced lower levels of AE. In conclusion, K7 and AE cannot be used to differentiate TCC from prostate carcinoma in dogs.