COORDINATED 3-DIMENSIONAL RECONSTRUCTION FROM SERIAL SECTIONS AT MACROSCOPIC AND MICROSCOPIC LEVELS OF RESOLUTION - THE HUMAN-HEART
COORDINATED 3-DIMENSIONAL RECONSTRUCTION FROM SERIAL SECTIONS AT MACROSCOPIC AND MICROSCOPIC LEVELS OF RESOLUTION - THE HUMAN-HEART
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DOI：
10.1002/ar.1092190415
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发表时间：
1987-12-01
影响因子：
--
通讯作者：
PROTHERO, J
中科院分区：
文献类型：
--
作者：
MCLEAN, MR;PROTHERO, J
这份报告描述了我们为在两个非常不同的分辨率水平上获得相关的定量结构信息而开发的程序。利用计算机辅助技术，以正确的标量和地形关系准确地重建了整个器官和器官内的组织样本。一种特殊设计的切割器--大振动刀，被用来连续地从宏观上切开人体的脑室。每一片都拍了照片。从左室壁特定位置的切片上切除的组织块被嵌入塑料中；连续的3µm切片在两个垂直方向中的每个方向上被切割。用半自动显微镜拍摄显微照片。宏观和微观切片的图像都被投影到位板上，并手动数字化。得到的x、y和z坐标表格在VAX 11/750计算机上重新组合，然后传输到高性能图形工作站并以三维图像的形式显示。显微图像显示在相对于宏观(母体)结构的正确参照系中。
This report describes procedures we have developed for obtaining correlated quantitative structural information at two very different levels of resolution. Accurate reconstructions of entire organs and samples of tissue within organs were produced in correct scalar and topographical relationship using computer-assisted techniques. A specially designed sectioning apparatus, a macrovibratome, was used to section serially the ventricles of the human heart macroscopically. Photographs were taken of every slice. A tissue block excised from a slice at a specified locus in the left ventricular wall was embedded in plastic; serial-3 .mu.m sections were cut in each of two orthogonal orientations. Photomicrographs were taken by semi-automated microscopy. Images of both macroscopic and microscopic sections were projected onto a bitpad and manually digitized. The resulting tables of x-, y-, and z-coordinates were reassembled on a VAX 11/750 computer, then transferred to a high-performance graphics workstation and displayed as three-dimensional images. Microscopic images were shown in the correct reference frame with respect to the macroscopic (parent) structure.