LIGHT enhances the bactericidal activity of human monocytes and neutrophils via HVEM
LIGHT enhances the bactericidal activity of human monocytes and neutrophils via HVEM
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DOI：
10.1189/jlb.1104694
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发表时间：
2006-02-01
影响因子：
5.5
通讯作者：
Kim, Byung-Sam
中科院分区：
文献类型：
--
作者：
Heo, Sook-Kyoung;Ju, Seong-A;Kim, Byung-Sam
人单核细胞和中性粒细胞在清除人体血液和组织中的细菌中起主要作用。我们发现，疱疹病毒进入介体（HVEM）在单核细胞和中性粒细胞中高度表达，其与“与细菌毒素同源，显示可诱导表达，并与单纯疱疹病毒糖蛋白D竞争HVEM/肿瘤坏死因子（TNF）相关2”（LIGHT）增强对单核细胞增多性李斯特菌和金黄色葡萄球菌的杀菌活性。LIGHT-HVEM相互作用增加了吞噬作用、白细胞介素（IL）-8的水平。单核细胞和中性粒细胞中的TNF-α、一氧化氮（NO）和活性氧（ROS）。抗HVEM单克隆抗体能够阻断LIGHT诱导的杀菌活性、细胞因子产生（IL-8和TNF-α）和ROS产生。此外，抑制ROS和NO的产生阻断了光诱导的杀菌活性。我们的研究结果表明，单核细胞和中性粒细胞中的LIGHT/HVEM相互作用有助于抗菌活性。
Human monocytes and neutrophils play major roles in clearing bacteria from human blood and tissues. We found that the herpes virus entry mediator (HVEM) was highly expressed in monocytes and neutrophils, and its interaction with "homologous to lymphotoxins, shows inducible expression, and competes with herpes simplex virus glycoprotein D for HVEM/tumor necrosis factor (TNF)-related 2" (LIGHT) enhanced bactericidal activity against Listeria monocytogenes and Staphylococcus aureus. The LIGHT-HVEM interaction increased levels of phagocytosis, interleukin (IL)-8. TNF-alpha, nitric oxide (NO), and reactive oxygen species (ROS) in monocytes and neutrophils. Anti-HVEM monoclonal antibody was able to block LIGHT-induced bactericidal activity, cytokine production (IL-8 and TNF-alpha), and ROS generation. Moreover, inhibition of ROS and NO production blocked LIGHT-induced bactericidal activity. Our results indicate that the LIGHT/HVEM interaction in monocytes and neutrophils contributes to antibacterial activity.