Antidepressant-like effect of flaxseed in rats exposed to chronic unpredictable stress
Antidepressant-like effect of flaxseed in rats exposed to chronic unpredictable stress
复制标题
亚麻籽对慢性不可预测应激大鼠的抗抑郁作用
DOI：
10.1002/brb3.1626
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发表时间：
2020
影响因子：
3.1
通讯作者：
Chen Anguo
中科院分区：
文献类型：
--
作者：
Han Yan;Deng Xin;Zhang Yi;Wang Xin;Zhu Xiongzhao;Mei Shiyong;Chen Anguo
抑郁症是一种严重的精神疾病。然而，很大一部分抑郁症患者在抗抑郁治疗后未能达到缓解。方法将大鼠随机分为5组：正常对照组（Sham-Sham）、慢性不可预知应激（CUS）+生理盐水组（S-SN）、CUS+艾司西酞普兰组（S-ESC）、CUS+亚麻籽油组（S-FO）和CUS+亚麻籽粉组（S-FF）。行为学测试采用蔗糖偏好实验和强迫游泳实验。采用酶联免疫吸附试验、真实的时间PCR和Western blot分别测定血清BDNF浓度、海马BDNF mRNA和蛋白表达。结果S-FO和S-FF大鼠的蔗糖偏好率显著高于S-SN和S-ESC大鼠（p < .01），而S-ESC大鼠的蔗糖偏好率显著低于Sham-Sham大鼠（p< .01）。S-FO和S-FF大鼠的不动时间显著短于S-SN大鼠（p< .01），S-ESC大鼠短于S-SN大鼠（p< .01）。S-FO、S-FF和S-ESC大鼠的血浆BDNF浓度显著低于Sham-Sham大鼠（p<0.01）; S-FO、S-FF和S-SN大鼠的BDNF浓度低于S-ESC大鼠。海马BDNF蛋白表达在S-ESC大鼠中显著高于S-SN大鼠（p<0.05）。S-ESC大鼠海马BDNF mRNA表达显著高于S-SN大鼠（p<0.01）。血浆和海马中BDNF基因表达与不动时间呈负相关（p< .05），而海马中BDNF基因表达与蔗糖偏好率呈正相关（p< .05）。结论亚麻籽油和亚麻籽粉对慢性应激大鼠具有抗抑郁样作用。亚麻籽可能对抑郁症有治疗作用。
IntroductionDepression is a serious mental illness. However, a significant proportion of patients with depression fail to achieve remission with antidepressant therapies. This study was conducted to explore the antidepressant‐like effect of flaxseed oil and flour in an animal model with depression‐like behaviors induced by chronic unpredictable stress (CUS).MethodsRats were randomly divided into five groups: normal control (Sham–Sham), CUS plus saline (S‐SN), CUS plus escitalopram (S‐Esc), CUS plus flaxseeds oil (S‐FO), and CUS plus flaxseed flour (S‐FF). Behaviors were tested using sucrose preference test and forced swimming test. The serum BDNF concentration, hippocampal BDNF mRNA, and protein expression were measured by enzyme‐linked immunosorbent assay, real‐time PCR, and Western blot, respectively.ResultsThe sucrose preference rate was significantly higher in S‐FO and S‐FF rats than in S‐SN and S‐Esc rats (p< .01), and lower in S‐Esc rats than in Sham–Sham rats (p< .01). The immobility time was significantly shorter in S‐FO and S‐FF rats than in S‐SN rats (p< .01), and shorter in S‐Esc rats than in S‐SN rats (p< .01). Plasma BDNF concentrations were significantly lower in S‐FO, S‐FF, and S‐Esc rats than in Sham–Sham rats (p< .01); BDNF was lower in S‐FO, S‐FF, and S‐SN rats than in S‐Esc rats. The hippocampal BDNF protein expression was significantly higher in S‐Esc rats than in S‐SN rats (p< .05). The hippocampal BDNF mRNA expression was significantly higher in S‐Esc rats than in S‐SN rats (p< .01). TheBDNFgene expression in plasma and the hippocampus negatively correlated with the immobility time (p< .05), butBDNFgene expression in the hippocampus positively correlated with the sucrose preference rate (p< .05).ConclusionFlaxseed oil and flaxseed flour exert antidepressant‐like effect in rats exposed to chronic stress. Flaxseed may have a therapeutic effect on depression.