Dregea volubilis ameliorates concanavalin A-induced liver injury by facilitating apoptosis of activated T cells
Dregea volubilis ameliorates concanavalin A-induced liver injury by facilitating apoptosis of activated T cells
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Dregea volubilis 通过促进活化 T 细胞凋亡来改善刀豆蛋白 A 诱导的肝损伤
DOI：
10.3181/0801-rm-15
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发表时间：
2008-09-01
影响因子：
3.2
通讯作者：
Xu, Qiang
中科院分区：
文献类型：
--
作者：
Gong, Fangyuan;Shen, Yan;Xu, Qiang
T细胞活化是刀豆球蛋白A（ConA）诱导的肝损伤发病机制中的关键事件，促进活化T细胞凋亡可能为治疗提供策略。在这里，我们发现，从Dregea volubilis（DVE）的干部分的乙醇提取物抑制细胞增殖和诱导凋亡，这是选择性的刀豆蛋白A激活，而不是非激活，淋巴结细胞。给药DVE可预防Con A诱导的小鼠血清转氨酶升高、肝坏死和小鼠血清中TNF-α、IFN-γ、IL-2和IL-4升高。DVE还引起体内活化的T细胞凋亡。此外，在DVE处理的细胞中发现caspase-3活性片段增加。但DVE诱导的凋亡是Fas非依赖性的，因为它仍然在Fas配体突变的gld/gld小鼠的T细胞中观察到。这些结果表明，DVE可能具有通过促进活化T细胞凋亡来治疗T细胞介导的疾病的巨大潜力。
Activation of T cells is a critical event in the pathogenesis of concanavalin A (Con A)-induced liver injury, and facilitating apoptosis of activated T cells may provide a strategy for the treatment. Here, we found that the ethanol extract from the stem parts of Dregea volubilis (DVE) inhibited cell proliferation and induced apoptosis, which was selective for Con A-activated, rather than nonactivated, lymph node cells. Administration of DVE prevented mice from Con A-induced elevation of serum transaminases, liver necrosis and increased TNF-alpha, IFN-gamma, IL-2 and IL-4 in mice sera. DVE also caused apoptosis of in vivo activated T cells. In addition, increased active fragments of caspase-3 were found in the DVE-treated cells. But DVE-induced apoptosis was Fas-independent, as it was still observed in T cells from Fas ligand-mutated gld/gld mice. These results suggest that DVE may have great potential to treat T cell-mediated diseases through facilitating apoptosis of activated T cells.