Magnesium sulfate reduces inflammation-associated brain injury in fetal mice.
Magnesium sulfate reduces inflammation-associated brain injury in fetal mice.
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DOI：
10.1016/j.ajog.2010.01.022
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发表时间：
2010-03
影响因子：
9.8
通讯作者：
Elovitz MA
中科院分区：
文献类型：
--
作者：
Burd I;Breen K;Friedman A;Chai J;Elovitz MA
研究硫酸镁 (MgSO4) 是否可以预防炎症相关早产 (PTB) 中的胎儿脑损伤。利用 PTB、LPS 或通过宫内注射暴露于生理盐水 (NS) 的小鼠模型，随机接受 MgSO4 或 NS 腹膜内治疗。从4个治疗组来看，1）NS+NS； 2)LPS+NS； 3)LPS+MgSO4； 4)NS+MgSO4，收集胎儿大脑用于QPCR研究和原代神经元培养。评估了细胞因子的 mRNA 表达、细胞死亡以及神经元和神经胶质分化的标志物。进行了免疫细胞化学和共聚焦显微镜检查。 LPS+NS组和LPS+MgSO4组促炎细胞因子、细胞死亡标志物以及促少突胶质细胞和星形胶质细胞发育标志物的表达均无差异（均P>0.05）。 LPS+NS 的神经元培养物表现出形态学变化，并且 MgSO4 可以防止这种神经元损伤（P<0.001）。改善炎症相关 PTB 中的神经元损伤可能是 MgSO4 预防脑瘫的关键机制。
To investigate whether magnesium sulfate (MgSO4) prevents fetal brain injury in inflammation-associated preterm birth (PTB). Utilising a mouse model of PTB, LPS or normal saline (NS)-exposed mice via intrauterine injection, were randomized to intraperitoneal treatment with MgSO4 or NS. From the 4 treatment groups, 1)NS+NS; 2)LPS+NS; 3)LPS+MgSO4; and 4)NS+MgSO4, fetal brains were collected for QPCR studies and primary neuronal cultures. mRNA expression of cytokines, cell death, and markers of neuronal and glial differentiation were assessed. Immunocytochemistry and confocal microscopy were performed. There was no difference between LPS+NS and LPS+MgSO4 groups in expression of pro-inflammatory cytokines, cell death markers as well markers of pro-oligodendrocyte and astrocyte development (P>0.05 for all). Neuronal cultures from LPS+NS demonstrated morphological changes and this neuronal injury was prevented by MgSO4 (P<0.001). Amelioration of neuronal injury in inflammation-associated PTB may be a key mechanism by which MgSO4 prevents cerebral palsy.
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