Mitogen-activated protein kinase kinase 7 is an activator of the c-Jun NH2-terminal kinase
Mitogen-activated protein kinase kinase 7 is an activator of the c-Jun NH2-terminal kinase
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DOI：
10.1073/pnas.94.14.7337
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发表时间：
1997-07-08
影响因子：
11.1
通讯作者：
Davis, RJ
中科院分区：
文献类型：
--
作者：
Tournier, C;Whitmarsh, AJ;Davis, RJ
丝裂原活化蛋白（MAP）激酶的c-Jun NH 2-末端激酶（JNK）组通过Thr和Tyr上的磷酸化激活。在这里，我们报告的哺乳动物MAP激酶激酶组（MKK 7），作为JNK的激活剂的功能的一个新成员的分子克隆。体外蛋白激酶测定表明，MKK 7磷酸化和激活JNK，但不是MAP激酶的p38或细胞外信号调节激酶组。MKK 7在培养细胞中的表达引起JNK信号转导途径的激活。因此，MKK 7被确定为JNK信号转导途径的新组分。
The c-Jun NH2-terminal kinase (JNK) group of mitogen-activated protein (MAP) kinases is activated by phosphorylation on Thr and Tyr. Here we report the molecular cloning of a new member of the mammalian MAP kinase kinase group (MKK7) that functions as an activator of JNK. In vitro protein kinase assays demonstrate that MKK7 phosphorylates and activates JNK, but not the p38 or extracellular signal-regulated kinase groups of MAP kinase. Expression of MKK7 in cultured cells causes activation of the JNK signal transduction pathway. MKK7 is therefore established to be a novel component of the JNK signal transduction pathway.