The Na+,K+/H+-antiporter Nha1 influences the plasma membrane potential of Saccharomyces cerevisiae
The Na+,K+/H+-antiporter Nha1 influences the plasma membrane potential of Saccharomyces cerevisiae
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DOI：
10.1111/j.1567-1364.2006.00062.x
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发表时间：
2006-08-01
影响因子：
3.2
通讯作者：
Sychrova, Hana
中科院分区：
文献类型：
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作者：
Kinclova-Zimmermannova, Olga;Gaskova, Dana;Sychrova, Hana
酿酒酵母（Saccharomycescerevisiae）中存在三种不同的钠转运系统（Ena 1 -4p，Nha 1 p，Nhx 1 p）。他们的情况下对碱金属阳离子的耐受性和膜电位的影响进行了研究。这三种钠转运蛋白均参与Na+、Li+、K+和Cs+稳态的维持。荧光电位探针（diS-C-3（3）试验）在细胞悬液中分布的测量结果表明，缺乏所有三种转运蛋白会使质膜去极化。Na ~+，K ~+/H ~+逆向转运蛋白Nha 1的过表达导致质膜超极化，从而增加了对Cs ~+，Tl ~+和潮霉素B的敏感性。这是第一个证据表明，活性的Na+，K+/H+逆向转运蛋白可以发挥作用，在酵母细胞的质膜电位的稳态调节。
There are three different sodium transport systems (Ena1-4p, Nha1p, Nhx1p) in Saccharomyces cerevisiae. The effect of their absence on the tolerance to alkali-metal cations and on the membrane potential was studied. All three sodium transporters were found to participate in the maintenance of Na+, Li+, K+ and Cs+ homeostasis. Measurements of the distribution of a fluorescent potentiometric probe (diS-C-3(3) assay) in cell suspensions showed that the lack of all three transporters depolarizes the plasma membrane. The overexpression of the Na+,K+/H+ antiporter Nha1 resulted in the hyperpolarization of the plasma membrane and consequently increased the sensitivity to Cs+, Tl+ and hygromycin B. This is the first evidence that the activity of a Na+,K+/H+ antiporter could play a role in the homeostatic regulation of the plasma membrane potential in yeast cells.