Continuous volumetric imaging via an optical phase-locked ultrasound lens.
Continuous volumetric imaging via an optical phase-locked ultrasound lens.
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DOI：
10.1038/nmeth.3476
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发表时间：
2015-08
期刊：
影响因子：
48
通讯作者：
Cui M
中科院分区：
文献类型：
--
作者：
Kong L;Tang J;Little JP;Yu Y;Lämmermann T;Lin CP;Germain RN;Cui M
高时空分辨率的体内成像是理解复杂生物系统的关键。将光学锁相超声镜头集成到传统的双光子荧光显微镜中，我们实现了微秒级的轴向扫描，从而实现了高速体积成像。我们将该系统应用于快速过程的多色体积成像，包括行为小鼠大脑皮层中的钙动态，以及免疫细胞的瞬时形态变化和运输。
In vivo imaging at high spatiotemporal resolution holds the key to the fundamental understanding of complex biological systems. Integrating an optical phase-locked ultrasound lens into a conventional two-photon fluorescence microscope, we achieved microsecond scale axial scanning, which enabled high-speed volumetric imaging. We applied this system to multicolor volumetric imaging of fast processes, including calcium dynamics in the cerebral cortex of behaving mice, and transient morphology changes and trafficking of immune cells.