Tuning cell adhesion on supported lipid bilayers via nanoscale geometry
Tuning cell adhesion on supported lipid bilayers via nanoscale geometry
复制标题
通过纳米级几何结构调节支持的脂质双层上的细胞粘附
DOI：
10.1039/d1sm01407b
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发表时间：
2021-10-20
期刊：
影响因子：
3.4
通讯作者：
Hu, Jinglei
中科院分区：
文献类型：
--
作者：
Li, Long;Gao, Jie;Hu, Jinglei
细胞支持的脂质双层(SLB)黏附系统已被广泛用作研究膜界面受体-配体相互作用的模型系统。配体功能化的SLB可以直接沉积在固体或聚合物衬垫上。由此产生的一个重要问题是，SLB的几何形状是否会影响细胞黏附受体与配体的结合。通过介观力学模型和蒙特卡罗模拟，我们研究了波纹或蛋盒形状的SLB与液膜的粘附性。我们发现SLB的纳米尺度几何形状强烈影响受体与配体的结合。这种效应是由于黏附膜根据SLB的几何形状弯曲，以便黏附受体与配体结合。膜弯曲与受体-配体复合体的空间分布和膜的热波动相耦合。我们的结果表明，细胞与SLB的黏附可以通过调节SLB的纳米级几何形状来控制，这可能对未来组织工程和再生医学的发展具有深远的意义。
The cell-supported lipid bilayer (SLB) adhesion system has been widely used as the model system to study the receptor-ligand interactions that occur at the membrane interface. The ligand-functionalized SLBs are deposited either directly on solids or on polymer cushions. An important question that arises is whether the geometry of the SLB affects the binding of cell adhesion receptors to the ligands. By using a mesoscopic mechanical model and Monte Carlo simulations, we have investigated the adhesion of a fluid membrane to a corrugated or egg-carton shaped SLB. We find that the nanoscale geometry of the SLB strongly affects the receptor-ligand binding. This effect results from the fact that the adhering membrane bends according to the SLB geometry in order for the adhesion receptors to bind ligands. The membrane bending couples with spatial distribution of the receptor-ligand complexes and membrane thermal undulations. Our results demonstrate that cell adhesion to SLBs can be controlled by tuning the nanoscale geometry of the SLB, and may have profound implications for future development of tissue engineering and regenerative medicine.