A retrievable, water-soluble and biocompatible fluorescent probe for recognition of Cu(II) and sulfide based on a peptide receptor
A retrievable, water-soluble and biocompatible fluorescent probe for recognition of Cu(II) and sulfide based on a peptide receptor
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一种可回收、水溶性和生物相容性荧光探针，用于基于肽受体识别 Cu(II) 和硫化物
DOI：
10.1016/j.talanta.2015.04.075
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发表时间：
2015-10-01
期刊：
影响因子：
6.1
通讯作者：
Xu, Maotian
中科院分区：
文献类型：
--
作者：
Hao, Yuanqiang;Chen, Wansong;Xu, Maotian
设计并合成了一种识别Cu ~（2+）和S ~（2-）的新型荧光探针C-GGH。在100%水溶液中，C-GGH对Cu ~（2+）具有高选择性的ON-OFF型荧光响应。此外，原位生成的非荧光C-GGH-Cu ~（2+）配合物可作为一种有效的选择性传感硫离子的关-开型荧光探针。由于S ~（2-）与Cu ~（2+）的强结合力，硫阴离子可将Cu ~（2+）从C-GGH-Cu ~（2+）络合物中萃取出来，使C-GGH释放，使体系的荧光发射恢复。利用发射光谱、核磁共振、质谱和高效液相色谱等技术对C-GGH-Cu ~（2+）传感硫离子的置换机理进行了研究。探针C-GGH-Cu 2+允许检测缓冲水溶液中的S2-，LOD（检测限）为0.44 μ M。该传感器利用天然三肽GGH的优点，具有良好的生物相容性和水溶性。细胞成像研究表明，该探针可用于检测生物体系中的硫阴离子。(C)2015年由Elsevier B. V.出版
A novel fluorescent probe C-GGH for recognition of Cu2+ and S2- was designed and synthesized by incorporation a natural tripeptide GGH (Gly-Gly-His) moiety, which acts as a recognition unit for Cu2+, to a coumarin fluorophore. C-GGH displayed ON-OFF type fluorescence response towards Cu2+ with high selectivity in 100% aqueous solution. Furthermore, the in situ generated non-fluorekent C-GGH-Cu2+ complex can serve as an effective OFF-ON type fluorescent probe for selective sensing sulfide anion. Due to the strong binding affinity of S2- to Cu2+, sulfide anion can extract Cu2+ from C-GGH-Cu2+ complex, resulting the release of C-GGH and the revival of fluorescent emission of the system. Techniques of emission spectrometry, NMR, mass spectrometry, and HPLC were used to confirm the displacement mechanism of C-GGH-Cu2+ for sensing sulfide anion. The probe C-GGH-Cu2+ allowed detection of S2- in aqueous buffer solution with a LOD (limit of detection) of 0.44 mu M. By taking advantages of the natural tripeptide GGH, the sensor exhibits excellent biocompatibility and water solubility. Moreover, the cell imaging study demonstrated that the probe can be exploited to detect sulfur anion in biological systems. (C) 2015 Published by Elsevier B.V.