Raman-assisted crystallography of biomolecules at the synchrotron: instrumentation, methods and applications.
Raman-assisted crystallography of biomolecules at the synchrotron: instrumentation, methods and applications.
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DOI：
10.1016/j.bbapap.2010.07.021
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发表时间：
2011-06
期刊：
影响因子：
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通讯作者：
J. McGeehan;D. Bourgeois;A. Royant;P. Carpentier
中科院分区：
文献类型：
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作者：
J. McGeehan;D. Bourgeois;A. Royant;P. Carpentier
拉曼光谱是一种强大的技术，近年来已成功与 X 射线晶体学结合用于生物大分子系统的分析。两种技术之间的互补性在多个阶段得到说明，包括样品制备、数据收集和从机械角度进行结构解释。描述了仪器的当前状态，重点是基于同步加速器的设置。参考最近处理金属蛋白、光敏蛋白和氧化还原蛋白的例子，回顾了拉曼显微分光光度法的当前和未来应用。讨论了拉曼显微分光光度法评估 X 辐射损伤的附加价值，并强调了其在研究结晶 DNA 分子方面的适用性。
Raman spectroscopy is a powerful technique that, in recent years, has been successfully coupled to X-ray crystallography for the analysis of biological macromolecular systems. The complementarity between both techniques is illustrated at multiple stages, including sample preparation, data collection and structural interpretation with a mechanistic perspective. The current state of instrumentation is described, focusing on synchrotron based setups. Present and future applications of Raman microspectrophotometry are reviewed with reference to recent examples dealing with metallo-, photosensitive-, and redox-proteins. The added value of Raman microspectrophotometry to assess X-radiation damage is discussed, and its applicability to investigate crystalline DNA molecules is also emphasized.