SEQUENCE HETEROGENEITY, MULTIPLICITY, AND GENOMIC ORGANIZATION OF ALPHA-TUBULIN AND BETA-TUBULIN GENES IN SEA-URCHINS
SEQUENCE HETEROGENEITY, MULTIPLICITY, AND GENOMIC ORGANIZATION OF ALPHA-TUBULIN AND BETA-TUBULIN GENES IN SEA-URCHINS
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DOI：
10.1128/mcb.1.12.1125
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发表时间：
1981-01-01
影响因子：
5.3
通讯作者：
RUDERMAN, JV
中科院分区：
文献类型：
--
作者：
ALEXANDRAKI, D;RUDERMAN, JV
α编码基因的多样性、异质性和组织性。和度量。利用克隆（c[互补]DNA）和基因组微管蛋白序列对海胆（l.p octus）微管蛋白进行了分析。以未成熟生精睾丸poly(A)-containing RNA为模板构建cDNA克隆。-和。beta。-通过杂交选择和体外翻译相应mRNA鉴定微管蛋白克隆，然后对翻译产物进行免疫沉淀和二维凝胶电泳。.alpha。cDNA克隆包含一个序列，该序列编码。α的48个c端氨基酸。微管蛋白和mRNA 3‘’非翻译部分的104个碱基对。度量。cDNA插入包含. β的100个c端氨基酸的编码序列。微管蛋白和3”非编码序列的83个碱基对。在不同标准下进行的杂交选择表明存在几种异质的、密切相关的微管蛋白mRNA，表明存在异质的α。-和。beta。微管蛋白基因。每个微管蛋白基因家族/单倍体基因组至少有9-13个序列。将cDNA探针与全基因组DNA和克隆的种系DNA片段杂交后，没有证据表明.alpha具有密切的物理联系。-微管蛋白基因。DNA水平上的微管蛋白基因同一家族中的一些基因可能是聚集在一起的。
The multiplicity, heterogeneity and organization of the genes encoding the .alpha. and .beta. tubulins were analyzed in the sea urchin L. pictus by using cloned (c [complementary] DNA) and genomic tubulin sequences. cDNA clones were constructed by using immature spermatogenic testis poly(A)-containing RNA as a template. .alpha.- and .beta.-Tubulin clones were identified by hybrid selection and in vitro translation of the corresponding mRNA, followed by immunoprecipitation and 2-dimensional gel electrophoresis of the translation products. The .alpha. cDNA clone contains a sequence that encodes the 48 C-terminal amino acids of .alpha. tubulin and 104 base pairs of the 3'' nontranslated portion of the mRNA. The .beta. cDNA insertion contains the coding sequence for the 100 C-terminal amino acids of .beta. tubulin and 83 base pairs of the 3'' noncoding sequence. Hybrid selections performed at different criteria demonstrated the presence of several heterogeneous, closely related tubulin mRNA, suggesting the existence of heterogeneous .alpha.- and .beta.-tubulin genes. There are at least 9-13 sequences for each of the 2 tubulin gene families/haploid genome. Hybridization of the cDNA probes to both total genomic DNA and cloned germline DNA fragments gave no evidence for close physical linkage of .alpha.-tubulin genes with .beta.-tubulin genes at the DNA level. Some genes within the same family are probably clustered.