Homology between a histidine-rich protein from Plasmodium lophurae and a protein associated with the knob-like protrusions on membranes of erythrocytes infected with Plasmodium falciparum.
Homology between a histidine-rich protein from Plasmodium lophurae and a protein associated with the knob-like protrusions on membranes of erythrocytes infected with Plasmodium falciparum.
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DOI：
10.1084/jem.151.6.1534
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发表时间：
1980-06-01
影响因子：
15.3
通讯作者：
Kilejian, A
中科院分区：
文献类型：
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作者：
Kilejian, A
比较了几种放射性氨基酸掺入恶性疟原虫球蛋白的情况。组氨酸比脯氨酸表现出更好的掺入。蛋白质水解产物，其中除了组氨酸和甲硫氨酸的所有主要氨基酸，表现出相对较差的掺入相比，脯氨酸，没有标记可以检测到与甲硫氨酸或亮氨酸。这些结果有力地表明，节蛋白的氨基酸组成具有相同的特性，从P. lophurae的富含组氨酸的蛋白质的特征。免疫电子显微镜表明这两种蛋白质之间可能存在免疫交叉反应。
The incorporation of several radioactive amino acids into the knob protein of Plasmodium falciparum was compared. Histidine showed better incorporation than proline. A protein hydrolysate, which had all major amino acids except histidine and methionine, showed relatively poor incorporation as compared with proline, and no labeling could be detected with methionine or leucine. These results strongly suggest that the amino acid composition of the knob protein has the same peculiarities as that of a histidine-rich protein characterized from P. lophurae. Immunoelectron microscopy suggested possible immunological cross-reactivity between these two proteins.