Reovirus, isolated from SARS patients
Reovirus, isolated from SARS patients
复制标题
DOI：
10.1360/03wc0294
复制
发表时间：
2003-07-01
影响因子：
--
通讯作者：
Zhang, XM
中科院分区：
文献类型：
--
作者：
Duan, Q;Zhu, H;Zhang, XM
北京受到SARS的严重影响，SARS相关的冠状病毒已被证实是其原因。然而，临床和实验证据暗示了合并感染的可能性。本报告从北京首例SARS患者及其母亲的咽拭子中分离到呼肠孤病毒。电镜鉴定显示呼肠孤病毒的特征。38例SARS患者标本中有24例呼肠孤病毒阳性。为呼肠孤病毒设计的引物扩增了几个DNA片段，对其中一个片段（S2基因片段）进行了测序，这表明它是一种独特的呼肠孤病毒（正呼肠孤病毒）。初步的动物实验表明，接种呼肠孤病毒的小鼠可引起非典型肺炎死亡。然而，呼肠孤病毒与SARS爆发的关系需要进一步研究。
Beijing has been severely affected by SARS, and SARS-associated coronavirus has been confirmed as its cause. However, clinical and experimental evidence implicates the possibility of co-infection. In this report, reovirus was isolated from throat swabs of SARS patients, including the first case in Beijing and her mother. Identification with the electron microscopy revealed the characteristic features of reovirus. 24 of 38 samples from other SARS cases were found to have serologic responses to the reovirus. Primers designed for reovirus have amplified several fragments of DNA, one of which was sequenced (S2 gene fragment), which indicates it as a unique reovirus (orthoreovirus). Preliminary animal experiment showed that inoculation of the reovirus in mice caused death with atypical pneumonia. Nevertheless, the association of reovirus with SARS outbreak requires to be further investigated.