Fosfomycin resistance among vancomycin-resistant enterococci owing to transfer of a plasmid harbouring the fosB gene
Fosfomycin resistance among vancomycin-resistant enterococci owing to transfer of a plasmid harbouring the fosB gene
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由于携带 fosB 基因的质粒的转移，耐万古霉素肠球菌对磷霉素产生耐药性
DOI：
10.1016/j.ijantimicag.2013.11.003
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发表时间：
2014-04-01
影响因子：
10.8
通讯作者：
Yu, Yun-song
中科院分区：
文献类型：
--
作者：
Qu, Ting-ting;Shi, Ke-ren;Yu, Yun-song
对浙江省临床分离的45株万古霉素耐药肠球菌（VRE）进行了质粒介导的磷霉素耐药决定簇的研究。19株VRE对磷霉素耐药，其中18株为接合型磷霉素耐药，fosB阳性。在其余分离株中未检测到报道的fos基因。在18个携带fosB的菌株中，fosB基因总是侧接tnpA，表明相同的新的fosB转座子。在18个携带fosB的分离株中，有10个分离株的fosB和tnpA基因被发现插入到vanA转座子Tn 1546中。在其余8个分离株的fosB和vanA基因位于不同的质粒。这些结果表明，收购的接合质粒窝藏新的fosB转座子（ISL 3-like转座子）和Tn 1546-like转座子（含有vanA和fosB）可以解释，至少部分，最近增加磷霉素耐药屎肠球菌在中国。(C)2013年Elsevier B. V.和国际化疗学会。All rights reserved.
The presence and characterisation of plasmid-mediated fosfomycin resistance determinants were investigated among 45 clinical vancomycin-resistant enterococci (VRE) isolated in Zhejiang Province, China. In total, 19 VRE were resistant to fosfomycin, of which 18 isolates had conjugative fosfomycin resistance and were positive for fosB. No reported fos genes were detected in the remaining isolate. Among the 18 fosB-carrying isolates, the fosB gene was always flanked by tnpA, suggesting the same novel fosB transposon. In 10 of the 18 fosB-carrying isolates, the fosB and tnpA genes were found reversely inserted in the vanA transposon Tn1546. In the remaining eight isolates the fosB and vanA genes were located on different plasmids. These findings indicate that acquisition of the conjugative plasmid harbouring the novel fosB transposon (ISL3-like transposon) and the Tn1546-like transposon (containing vanA and fosB) may explain, at least in part, the recent increase in fosfomycin-resistant Enterococcus faecium in China. (C) 2013 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.