Characterization of agglutinin-like sequence genes from non-albicans Candida and phylogenetic analysis of the ALS family.
Characterization of agglutinin-like sequence genes from non-albicans Candida and phylogenetic analysis of the ALS family.
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非白色念珠菌凝集素样序列基因的表征和 ALS 家族的系统发育分析。
DOI：
10.1093/genetics/157.4.1555
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发表时间：
2001
期刊：
影响因子：
3.3
通讯作者：
Arnold,J
中科院分区：
文献类型：
--
作者：
Hoyer,LL;Fundyga,R;Hecht,JE;Kapteyn,JC;Klis,FM;Arnold,J
白念珠菌的ALS（凝集素样序列）基因家族编码细胞表面糖蛋白，这些糖蛋白与生物体与宿主表面的粘附有关。用ALS特异性探针进行Southern印迹分析，结果表明，在C.都柏林和C. tropicalis;从每种生物体中分离出三个部分ALS基因。北方印迹分析表明，C.白色假丝酵母C.都柏林则不同。用抗Als血清进行的Western印迹显示，在每种非白念珠菌的细胞壁中，交叉反应蛋白通过β 1，6-葡聚糖连接，表明这些生物体中Als蛋白的相似细胞壁结构和保守加工。虽然ALS家族存在于每种生物体中，但C.白色念珠菌、C. dubliniensis和C. tropicalisALS基因表明，在每个物种内，序列多样化是广泛的，并且出现了独特的ALS序列。对ALS和SAP（分泌型胰蛋白酶）家族的系统发育分析表明，ALS家族比SAP家族年轻。C.白色念珠菌、C. dubliniensis和C. tropicalis倾向于位于同样编码SAP家族基因的染色体上，然而这两个家族却有着意想不到的不同进化历史。ALS基因中存在的串联重复序列之间的同源重组可以解释在一个主要的克隆生物体中，如C。白色念珠菌。
The ALS (agglutinin-likesequence) gene family ofCandida albicansencodes cell-surface glycoproteins implicated in adhesion of the organism to host surfaces. Southern blot analysis with ALS-specific probes suggested the presence of ALS gene families inC. dubliniensisandC. tropicalis; three partial ALS genes were isolated from each organism. Northern blot analysis demonstrated that mechanisms governing expression of ALS genes inC. albicansandC. dubliniensisare different. Western blots with an anti-Als serum showed that cross-reactive proteins are linked by β1,6-glucan in the cell wall of each non-albicansCandida, suggesting similar cell wall architecture and conserved processing of Als proteins in these organisms. Although an ALS family is present in each organism, phylogenetic analysis of theC. albicans, C. dubliniensis, andC. tropicalisALS genes indicated that, within each species, sequence diversification is extensive and unique ALS sequences have arisen. Phylogenetic analysis of the ALS and SAP (secretedaspartylproteinase) families show that the ALS family is younger than the SAP family. ALS genes inC. albicans, C. dubliniensis, andC. tropicalistend to be located on chromosomes that also encode genes from the SAP family, yet the two families have unexpectedly different evolutionary histories. Homologous recombination between the tandem repeat sequences present in ALS genes could explain the different histories for co-localized genes in a predominantly clonal organism likeC. albicans.