Simultaneous analysis of multiple urinary biomarkers for the evaluation of oxidative stress by automated online in-tube solid-phase microextraction coupled with negative/positive ion-switching mode liquid chromatography-tandem mass spectrometry.
Simultaneous analysis of multiple urinary biomarkers for the evaluation of oxidative stress by automated online in-tube solid-phase microextraction coupled with negative/positive ion-switching mode liquid chromatography-tandem mass spectrometry.
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通过自动在线管内固相微萃取结合负/正离子转换模式液相色谱-串联质谱法，同时分析多种尿液生物标志物，以评估氧化应激。
DOI：
10.1002/jssc.201800175
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发表时间：
2018
影响因子：
3.1
通讯作者：
Hiroyuki Kataoka
中科院分区：
文献类型：
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作者：
Akihiro Saito;Mariko Hamano;Hiroyuki Kataoka
建立了在线自动测定人尿中8-异前列腺素、8-羟基-2‘-脱氧鸟苷和3-硝基-L酪氨酸的方法。该方法包括管内固相微萃取，使用Carboxen 1006 PLOT毛细管柱作为萃取设备，然后使用CX柱进行液相色谱-串联质谱分析，并通过多反应监测在负/正切换离子模式下进行检测。使用稳定的同位素标记的内标，每个氧化应激生物标志物在0.02~2.0 ng/mL范围内都表现出良好的线性关系。最低检出限(S/N=0.03)为3.4~21.5pg/mL，日内和日间精密度(RSD)分别为3.9%和6.5%(n=0.5)。该方法成功地应用于尿样的分析，不需要任何其他前处理和干扰峰。
This study described an automated online method for the simultaneous determination of 8‐isoprostane, 8‐hydroxy‐2′‐deoxyguanosine, and 3‐nitro‐l‐tyrosine in human urine. The method involves in‐tube solid‐phase microextraction using a Carboxen 1006 PLOT capillary column as an extraction device, followed by liquid chromatography with tandem mass spectrometry using a CX column and detection in the negative/positive switching ion‐mode by multiple reaction monitoring. Using their stable isotope‐labeled internal standards, each of these oxidative stress biomarkers showed good linearity from 0.02 to 2.0 ng/mL. Their detection limits (S/N = 3) were 3.4–21.5 pg/mL, and their intra‐ and inter‐day precisions (relative standard deviations) were >3.9 and 6.5% (n= 5), respectively. This method was applied successfully to the analysis of urine samples, without any other pretreatment and interference peaks.