Homology of the rat basophilic leukemia cell and the rat mucosal mast cell.
Homology of the rat basophilic leukemia cell and the rat mucosal mast cell.
复制标题
大鼠嗜碱性白血病细胞和大鼠粘膜肥大细胞的同源性。
DOI：
10.1073/pnas.82.11.3871
复制
发表时间：
1985
影响因子：
11.1
通讯作者：
Woodbury,RG
中科院分区：
文献类型：
--
作者：
Seldin,DC;Adelman,S;Austen,KF;Stevens,RL;Hein,A;Caulfield,JP;Woodbury,RG
分泌颗粒的大鼠嗜碱性白血病（RBL-1）细胞，化学产生的肿瘤细胞系保持在组织培养中，被证明与阿辛蓝染色，但不与番红复染，并有稀疏，小，电子致密颗粒。一个Mr 25，000的蛋白质是RBL-1细胞提取物中主要的[3H]二异丙基氟磷酸结合蛋白。双免疫扩散分析的提取物显示免疫反应性大鼠肥大细胞蛋白酶（RMCP）-II，一个Mr 25,000中性蛋白酶存在于分泌颗粒的大鼠粘膜肥大细胞和培养的大鼠骨髓来源的肥大细胞，但没有免疫反应性RMCP-I，主要的中性蛋白酶的大鼠结缔组织肥大细胞。通过径向免疫扩散，每10（6）个细胞中有66.8 ng RMCP-II。而大鼠结缔组织肥大细胞用阿辛蓝和番红染色，并含有肝素蛋白聚糖，大鼠粘膜和大鼠骨髓来源的肥大细胞仅用阿辛蓝染色，并含有非肝素蛋白聚糖和较少量的组胺。大鼠粘膜肥大细胞在体内和大鼠骨髓来源的肥大细胞在体外的增殖需要T细胞因子，而没有类似的要求已被观察到结缔组织肥大细胞。转化的RBL-1肿瘤细胞，其生长不依赖于标准组织培养基中存在的因子以外的因子，先前已显示主要含有硫酸软骨素二-B蛋白聚糖和少量组胺。大鼠粘膜肥大细胞、大鼠培养物来源的肥大细胞和RBL-1细胞的相似组织学和分泌颗粒生物化学表明它们包括不同于大鼠结缔组织肥大细胞的单个肥大细胞亚类。
Secretory granules of the rat basophilic leukemia (RBL-1) cell, a chemically generated tumor cell line maintained in tissue culture, were shown to stain with alcian blue but not with safranin counterstain and to have sparse, small, electron-dense granules. A Mr 25,000 protein was the major [3H]diisopropyl fluorophosphate-binding protein in extracts of RBL-1 cells. Double-immunodiffusion analysis of extracts revealed immunoreactivity for rat mast cell protease (RMCP)-II, a Mr 25,000 neutral protease present in the secretory granules of rat mucosal mast cells and cultured rat bone marrow-derived mast cells, but no immunoreactivity for RMCP-I, the predominant neutral protease of rat connective tissue mast cells. By radial immunodiffusion, there was 66.8 ng of RMCP-II per 10(6) cells. Whereas rat connective tissue mast cells stain with alcian blue and safranin and contain heparin proteoglycan, rat mucosal and rat bone marrow-derived mast cells stain with alcian blue only and contain a non-heparin proteoglycan and lesser amounts of histamine. Proliferation of rat mucosal mast cells in vivo and rat bone marrow-derived mast cells in vitro requires T-cell factors, whereas no comparable requirement has been observed for connective tissue mast cells. The transformed RBL-1 tumor cells, whose growth is independent of factors other than those present in standard tissue culture medium, has previously been shown to contain predominantly chondroitin sulfate di-B proteoglycans and low amounts of histamine. The similar histology and secretory granule biochemistry of the rat mucosal mast cells, rat culture-derived mast cell, and RBL-1 cell suggest that they comprise a single mast cell subclass distinct from the rat connective tissue mast cell.