Build up of multilayered thin films with chitosan/DNA pairs on poly(D,L-lactic acid) films: Physical chemistry and sustained release behavior
Build up of multilayered thin films with chitosan/DNA pairs on poly(D,L-lactic acid) films: Physical chemistry and sustained release behavior
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在聚（D,L-乳酸）薄膜上用壳聚糖/DNA 对构建多层薄膜：物理化学和缓释行为
DOI：
10.1002/jbm.a.31458
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发表时间：
2008-02-01
影响因子：
4.9
通讯作者：
Yang, Li
中科院分区：
文献类型：
--
作者：
Cai, Kaiyong;Hu, Yan;Yang, Li
为了实现聚乳酸（PDLLA）的表面工程化，利用壳聚糖（Chi）和脱氧核糖核酸（DNA）的层层自组装（LbL）技术制备了多层膜。通过傅里叶变换红外光谱（FTIR），X射线光电子能谱（XPS），水接触角测量，和原子力显微镜（AFM），分别监测多层膜的形成。通过接触角测量显示，仅在第五次连续沉积（PEI/（DNA/Chi）（2））之后才形成完全覆盖的Chi/DNA对膜。多层膜的表面化学和形貌与相应的最外层成分直接相关。在PEI/（DNA/Chi）（5）/DNA层状PDLLA膜上观察到可辨别的岛状结构。溶菌酶介导的多层降解和DNA释放测量表明，DNA逐渐释放到孵育介质中长达32 h的时间。本文提出的方法可用于开发从生物医学材料和装置的表面原位控制施用功能性DNA构建体。(c)2007 Wiley Periodicals，Inc.
In an effort to surface engineering Of poiy(D,L-lactic acid) (PDLLA), layer-by-layer (LbL) self-assembly of chitosan (Chi) and deoxyribonucleic acid (DNA) were employed to build up multilayered films. The formation of multilayers was monitored by using Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), water contact-angle measurement, and atomic force microscopy (AFM), respectively. A full coverage of Chi/DNA pair film was formed only after the fifth sequential deposition (PEI/(DNA/Chi)(2)), which was revealed by contact-angle measurement. Surface chemistry and topography of multilayered films were directly related to the corresponding outmost layer component. Discernable island-like structures on PEI/(DNA/Chi)(5)/DNA layered PDLLA film was observed. Lysozyme-mediated multilayer degradation and DNA-releasing measurement suggested that DNA was gradually released into the incubation medium over a period of up to 32 h. The approach presented here may be exploited to develop controlled administration of functional DNA constructs from the surfaces of biomedical materials and devices in situ. (c) 2007 Wiley Periodicals, Inc.