Susceptibility to chemical insecticides of two Brazilian populations of the visceral leishmaniasis vector Lutzomyia longipalpis (Diptera: Psychodidae)
Susceptibility to chemical insecticides of two Brazilian populations of the visceral leishmaniasis vector Lutzomyia longipalpis (Diptera: Psychodidae)
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DOI：
10.1111/j.1365-3156.2009.02371.x
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发表时间：
2009-10-01
影响因子：
3.3
通讯作者：
Reithinger, R.
中科院分区：
文献类型：
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作者：
Alexander, B.;Barros, V. C.;Reithinger, R.
目的调查不同杀虫剂接触史（无接触史和重复接触史）的长掌卢蝇种群（Lapinha和Montes Claros）对杀虫剂的敏感性。方法(i)生物测定法监测暴露于多种杀虫剂的沙蝇长期存活情况；（ii）分析两个研究地点捕获的沙蝇个体的杀虫剂解毒酶水平。测试的杀虫剂为有机磷马拉硫磷和杀虫硫磷，以及拟除虫菊酯——高效氯氟氰菊酯、氯菊酯和溴氰菊酯。结果生存分析显示，尽管两种群对有机磷的敏感性总体上没有显著差异，但Lapinha沙蝇对拟除虫菊酯的敏感性明显高于Montes Claros沙蝇。多元回归分析还表明，不同地点对杀虫剂的敏感性随沙蝇性别的不同而不同。比较了两种沙蝇种群对杀虫剂的相对敏感性。因此，蒙氏沙蝇对马拉硫磷最敏感，其次是杀虫硫磷、溴氰菊酯和氯菊酯。对高效氯氟氰菊酯最敏感，其次是马拉硫磷、氯菊酯、溴氰菊酯和杀虫硫磷。生化分析表明，克拉氏沙蝇的杀虫剂解毒酶活性显著低于拉宾纳沙蝇。结论本研究结果首次记录了野生鹿群对杀虫剂敏感性显著降低的情况。longipalpis。它们证明了在喷洒计划之前和期间通过常规生物测定和微孔板测定来评估化学品对该物种的危害的重要性。
OBJECTIVES To investigate the insecticide susceptibility of two geographically separated Lutzomyia longipalpis populations (Lapinha and Montes Claros) with different histories of insecticide exposure (i.e. no exposure and repeated exposure, respectively).METHODS (i) Bioassay monitoring of sand fly survival over time when exposed to a range of insecticides; and (ii) analysis of the level of insecticide detoxification enzymes in individual sand flies caught at both study sites. Insecticides tested were the organophosphates malathion and fenitrothion and the pyrethroids lambda-cyhalothrin, permethrin and deltamethrin.RESULTS Survival analyses showed that whilst there was no overall significant difference in susceptibility of both populations to organophosphates, Lapinha sand flies were significantly more susceptible to pyrethroids than those from Montes Claros. Multiple regression analyses also showed that insecticide susceptibility in both locations varied with sand fly sex. The relative susceptibilities of the two sand fly populations to tested insecticides were also compared. Thus, Montes Claros sand flies were most susceptible to malathion, followed by fenitrothion, deltamethrin and permethrin. Those from Lapinha were most susceptible to lambda-cyhalothrin, followed by malathion, permethrin, deltamethrin and fenitrothion. Biochemical analyses demonstrated that Montes Claros sand flies had significantly lower insecticide detoxification enzyme activity than Lapinha sand flies.CONCLUSIONS Our results are the first record of significantly reduced susceptibility to the insecticides used in control of wild populations of Lu. longipalpis. They demonstrate the importance of evaluating chemicals against this species by conventional bioassay and microplate assays before and during spraying programmes.