Effect of Lemon Balm Water Extract on Fusarium Wilt Control in Strawberry and Antifungal Properties of Secondary Metabolites
Effect of Lemon Balm Water Extract on Fusarium Wilt Control in Strawberry and Antifungal Properties of Secondary Metabolites
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DOI：
10.2503/hortj.utd-121
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发表时间：
2020-01-01
影响因子：
1.2
通讯作者：
Matsubara, Yoh-ichi
中科院分区：
文献类型：
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作者：
Ahmad, Hasib;Matsubara, Yoh-ichi
本文研究了柠檬香油水提物对草莓枯萎病的体内防效及次生代谢物的抑菌性能。以草莓(Fragaria x ananassa Duch.，‘Sachinokal)为试材，用20%(w/v)柠檬香膏水提物处理草莓植株。接种尖孢镰刀菌f.sp.Fragariae(Fof)接种4周后，柠檬香精处理的植株地上部和根部的发病率和病情指数均较低。这些作用可以归因于根际土壤中的镰刀菌数量减少，这是由于提取物对根际土壤的抑菌和杀菌作用。结果表明，柠檬香精处理植株的地上部和根部干重均高于对照。根据超高效液相色谱-串联质谱仪(UPLC-MS/MS)的分析结果，迷迭香酸是水提物中浓度最高的代谢物，也是最稳定的代谢物。此外，通过体外抗真菌实验证实了迷迭香酸的抗真菌作用。因此，柠檬香油水提物具有抑制草莓枯萎病的作用，迷迭香酸是水提物中具有抗真菌活性的关键代谢产物之一。
In this study, the in vivo potential of lemon balm water extract on Fusarium wilt control in strawberry and the antifungal properties of secondary metabolites in the extract were investigated. Runner plants of strawberry (Fragaria x ananassa Duch., 'Sachinokal were treated with water extracts (20%, w/v) of lemon balm (Melissa officinalis L.) and inoculated with Fusarium oxysporum f. sp. fragariae (Fof). Four weeks after Fof inoculation, lower disease incidence and indices in both shoots and roots were observed in lemon balm-treated plants. These effects could be attributed to reduced Fusarium populations due to the fungistasis and fungicidal effects induced by the extract in the rhizospheric soil. Consequently, dry weights of shoots and roots in the plants treated with lemon balm extracts were higher than those of the control. Based on the results of ultra performance liquid chromatography-tandem mass spectrometer (UPLC-MS/MS) analyses, rosmarinic acid was the metabolite with the highest concentration and was also the most stable metabolite in the water extract. In addition, the antifungal effect of rosmarinic acid against Fof was confirmed by in vitro tests. Therefore, water extracts of lemon balm could suppress Fusarium wilt in strawberry plants and rosmarinic acid was one of the key metabolites with antifungal properties present in the water extract.