Woronin bodies move dynamically and bidirectionally by hitchhiking on early endosomes in Aspergillus nidulans.
Woronin bodies move dynamically and bidirectionally by hitchhiking on early endosomes in Aspergillus nidulans.
复制标题
Woronin 体通过搭便车在构巢曲霉的早期内体上进行动态和双向移动。
DOI：
10.1101/2023.01.20.524968
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Reck-Peterson,SamaraL
中科院分区：
文献类型：
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作者：
Songster,LiviaD;Bhuyan,Devahuti;Christensen,JennaR;Reck-Peterson,SamaraL
细胞器的正常功能取决于它们的细胞内定位，由细胞骨架轨道上的运动蛋白依赖性运输介导。过氧化物酶体不是直接与运动蛋白结合，而是通过搭便车在丝状真菌构巢曲霉的运动早期内体上移动。然而，过氧化物酶体搭便车的细胞功能尚不清楚。过氧化物酶体搭便车需要蛋白质 PxdA，该蛋白质在真菌亚门 Pezizomycotina 内保守，但在其他真菌分支中不存在。沃罗宁体是特殊的过氧化物酶体，也是盘菌亚门所独有的。在这些真菌中，多核菌丝片段被称为隔膜的不完整细胞壁分隔开，该细胞壁具有能够进行细胞质交换的中心孔。当菌丝段受损时，沃罗宁体会堵塞隔膜孔以防止大范围渗漏。在这里，我们测试了过氧化物酶体搭便车对于构巢曲霉中的沃罗宁身体运动、分布和功能是否重要。我们发现，Woronin 体蛋白存在于所有可运动的过氧化物酶体中，并在双向、长距离运动过程中搭在 PxdA 标记的早期内体上。通过敲除 pxdA 导致的过氧化物酶体搭便车损失显着影响了 Woronin 体在细胞质中的分布和运动，但 Woronin 体搭便车对于隔膜定位和堵塞最终是可有可无的。
The proper functioning of organelles depends on their intracellular localization, mediated by motor protein-dependent transport on cytoskeletal tracks. Rather than directly associating with a motor protein, peroxisomes move by hitchhiking on motile early endosomes in the filamentous fungus Aspergillus nidulans. However, the cellular function of peroxisome hitchhiking is unclear. Peroxisome hitchhiking requires the protein PxdA, which is conserved within the fungal subphylum Pezizomycotina, but absent from other fungal clades. Woronin bodies are specialized peroxisomes that are also unique to the Pezizomycotina. In these fungi, multinucleate hyphal segments are separated by incomplete cell walls called septa that possess a central pore enabling cytoplasmic exchange. Upon damage to a hyphal segment, Woronin bodies plug septal pores to prevent widespread leakage. Here, we tested if peroxisome hitchhiking is important for Woronin body motility, distribution, and function in A. nidulans. We show that Woronin body proteins are present within all motile peroxisomes and hitchhike on PxdA-labeled early endosomes during bidirectional, long-distance movements. Loss of peroxisome hitchhiking by knocking out pxdA significantly affected Woronin body distribution and motility in the cytoplasm, but Woronin body hitchhiking is ultimately dispensable for septal localization and plugging.