A HIGH CALORIE DIET INDUCES TYPE 2 DIABETES IN THE DESERT SAND RAT (PSAMMOMYS OBESUS)
A HIGH CALORIE DIET INDUCES TYPE 2 DIABETES IN THE DESERT SAND RAT (PSAMMOMYS OBESUS)
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DOI：
10.1170/108
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发表时间：
2007-01-01
影响因子：
1.6
通讯作者：
Griene, L.
中科院分区：
文献类型：
--
作者：
El Aoufi, S.;Gendre, P.;Griene, L.
2型糖尿病是心血管疾病的主要因素。慢性高血糖症的毒性作用涉及血管组织的许多改变，包括动脉粥样硬化。糖尿病综合征（DS-II）动脉粥样硬化的发病机制尚未完全阐明。更好地了解DS-II在主动脉水平的进展可以帮助我们识别动脉粥样硬化的抑制剂。研究表明，肥胖和高热量饮食（HCD）与DS-II的发展有关，然而，由于缺乏天然存在的DS-II实验模型，直接解决这些问题受到了影响。我们假设HCD诱导DS-II。本研究（持续15个月）旨在确定HCD是否诱导沙漠沙鼠（Psammomys obesus; P. obesus）中的DS-II。我们还评估了动物的主动脉的组织病理学与实验室饲料颗粒随意（高热量）和对照组（动物与自然发生的低热量饮食喂养;嗜盐植物）。在DS-II开发期间，定期（每周一次）评价体重和血液化学（葡萄糖、脂质和胰岛素水平），并每3个月评估一次这些动物的睾丸组织学，持续长达12个月。本研究表明，HCD中40%的动物在3个月时发生DS-II。组织学特征表明在动脉粥样硬化中观察到的典型改变，即，中膜层弹性纤维的改变以及胶原蛋白和葡糖胺聚糖的富集。本研究表明，肥胖大鼠是一个很好的动物模型，以研究DS-II的进展和动脉粥样硬化的发展。
Diabetes mellitus type 2 is a major factor for cardiovascular diseases. The toxic effects of chronic hyperglycemia involve many alterations in the vascular tissue, including atherosclerosis. The pathogenesis of atherosclerosis in the diabetic syndrome (DS-II) has not been fully elucidated. A better understanding of the progress of DS-II at the level of the aorta could help us to identify inhibitors of atherosclerosis. Studies have shown that obesity and high calorie diet (HCD) are associated with the development of DS-II, however the lack of naturally occurring experimental models of DS-II have impaired to directly address these issues. We hypothesize that a HCD induces DS-II. This study (15 months duration) is designed to determine if HCD induces DS-II in the desert sand rat (Psammomys obesus; P. obesus). We also evaluated the histopathology of the aorta in animals fed with a laboratory chow pellets ad libitum (hypercaloric) and in control (animals fed with the naturally occurring hypocaloric diet; halophile plants). The weight and blood chemistry (glucose, lipids, and insulin levels) were evaluated periodically (once per week), and the histology of the aortas of these animals were assessed every 3 months for up to 12 months, during the development of DS-II. This study demonstrates that 40% of the animals in HCD develop DS-II at 3 months. Histological characterization demonstrates the typical alterations observed in atherosclerosis, i.e., alteration of the elastic fibers of the media layer and enrichment in collagen and glucosaminoglycans. This study demonstrates that P. obesus is an excellent animal model to study the progression of DS-II and the development of atherosclerosis.