Magnifying stem cell lineages - The stop-EGFP mouse
Magnifying stem cell lineages - The stop-EGFP mouse
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DOI：
10.4161/cc.3.10.1202
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发表时间：
2004-10-01
期刊：
影响因子：
4.3
通讯作者：
Ro, S
中科院分区：
文献类型：
--
作者：
Ro, S
细胞命运图技术可以标记克隆细胞谱系，因为它们允许人们调查单个前体细胞产生的子代细胞的分布和类型。因此，可以在单细胞水平上研究前体细胞产生各种类型的后代细胞的潜力。Stop-EGFP转基因小鼠携带一个过早停止密码子的增强型绿色荧光蛋白(EGFP)基因，作为突变的靶基因。在过早终止密码子上发生突变的细胞及其后代细胞谱系将表达绿色荧光蛋白，因此可以利用荧光显微镜在体内追踪克隆细胞谱系。使用Stop-EGFP小鼠，可以在体内研究小鼠背部表皮中的干细胞克隆谱系，并可以随着时间的推移对相同的细胞谱系进行重复分析。使用Stop-EGFP小鼠进行的活体成像研究为了解表皮增殖单位(EPU)的结构提供了新的见解。STOP-EGFP系统为在发育研究和干细胞生物学中研究克隆细胞谱系提供了一种新的工具。
Cell fate mapping techniques which can label clonal cell lineages are of importance because they allow one to investigate the distribution and types of daughter cells arising from single precursor cells. Thus, the potential of precursor cells to generate various types of descendent cells can be studied at the single-cell level. The stop-EGFP transgenic mouse carries a premature stop codon-containing enhanced green fluorescent protein (EGFP) gene as a target gene for mutations. A cell having undergone a mutation at the premature stop codon and its descendant cell lineage will express EGFP, thus a clonal cell lineage can be traced in vivo using a fluorescent microscope. Using the stop-EGFP mouse, stem cell clonal lineages in the mouse dorsal epidermis can be investigated in vivo and repeated analyses of the same cell lineages can be performed over time. In vivo imaging studies possible with the stop-EGFP mouse provide new insights into the structure of epidermal proliferative units (EPUs). The stop-EGFP system provides a novel tool for investigating clonal cell lineages in developmental studies as well as in stem cell biology.