Multiplex-PCR for diagnosis of bacterial meningitis
Multiplex-PCR for diagnosis of bacterial meningitis
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DOI：
10.1007/s42770-019-00055-9
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发表时间：
2019-04-01
影响因子：
2.2
通讯作者：
Martinez, Marina Baquerizo
中科院分区：
文献类型：
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作者：
Albuquerque, Renata Chaves;Ramos Moreno, Ana Carolina;Martinez, Marina Baquerizo
考虑到脑膜炎的致命性和后遗症，快速诊断和及时治疗对患者的预后有很大影响。在这里，我们开发了一种多重PCR同时检测四个最流行的细菌病原体直接在CSF样本。多重PCR设计用于检测以下基因：fbsA（无乳链球菌）、lytA（肺炎链球菌）、crtA（脑膜炎奈瑟菌）、p6（流感嗜血杆菌）和16 S rRNA（任何细菌因子）。多重PCR的检测限为1 pg/L。在检测的447份CSF样本中，40份多重PCR阳性，其中27份细菌培养阳性，13份细菌培养阴性。我们的多重PCR是快速，可靠，易于实施到细菌性脑膜炎确认的实验室常规，特别是对于以前开始抗菌治疗的患者。我们的分子方法可以大大改善脑膜炎疾病负担的临床诊断和流行病学措施。
Considering the great lethality and sequels caused by meningitis, rapid diagnosis and prompt treatment initiation have a great impact on patient outcome. Here, we developed a multiplex-PCR for simultaneous detection of the four most prevalent bacterial pathogens directly in CSF samples. The multiplex-PCR was designed to detect the following genes: fbsA (Streptococcus agalactiae), lytA (Streptococcus pneumoniae), crtA (Neisseria meningitidis), p6 (Haemophilus influenzae), and 16S rRNA (any bacterial agent). The multiplex-PCR showed a DNA detection limit of 1pg/L. Among 447 CSF samples tested, 40 were multiplex-PCR positive, in which 27 and 13 had positive and negative bacterial culture, respectively. Our multiplex-PCR is fast, reliable, and easily implementable into a laboratory routine for bacterial meningitis confirmation, especially for patients who previously started antimicrobial therapy. Our molecular approach can substantially improve clinical diagnosis and epidemiological measures of meningitis disease burden.