MOLECULAR-CLONING AND DNA-SEQUENCE OF RAT AMELOGENIN AND A COMPARATIVE-ANALYSIS OF MAMMALIAN AMELOGENIN PROTEIN-SEQUENCE DIVERGENCE
MOLECULAR-CLONING AND DNA-SEQUENCE OF RAT AMELOGENIN AND A COMPARATIVE-ANALYSIS OF MAMMALIAN AMELOGENIN PROTEIN-SEQUENCE DIVERGENCE
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DOI：
10.1006/bbrc.1994.1109
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发表时间：
1994-01-28
影响因子：
3.1
通讯作者：
ROBINSON, C
中科院分区：
文献类型：
--
作者：
BONASS, WA;ROBINSON, PA;ROBINSON, C
发育中的大鼠门牙是牙釉质发育研究中常用的模型。研究大鼠牙釉质结构与该物种中主要发育的牙釉质蛋白序列之间的相关性是不可能的，因为迄今为止尚未报道大鼠釉原蛋白的 DNA 序列。本研究首次克隆了大鼠牙釉蛋白全长 cDNA 拷贝及其推导的一级序列。对该序列的详细分析提供了该基因通过内部序列复制进化的证据。将大鼠牙釉蛋白初级序列与其他物种公布的初级序列进行比较，证明这种蛋白质虽然表现出极高水平的序列保守性，但已经发生了显着的结构变化，这可能与不同哺乳动物群体牙釉质结构的改变有关。
The developing rat incisor is a common model used in the study of enamel development. It has been impossible to study correlation between rat enamel structure and the sequence of the major developing enamel protein in this species as to date a DNA sequence for rat amelogenin has not been reported. This study presents the first cloning of a full-length cDNA copy of rat amelogenin and its deduced primary sequence. Detailed analysis of this sequence provides evidence that the gene has evolved by internal sequence duplication. Comparison of the rat amelogenin primary sequence with those published for other species provides evidence that this protein, while exhibiting extreme levels of sequence conservation, has been subject to significant structural changes that may be related to alterations in enamel structure in different mammalian groups.