Transgene Introduction into the Chick Limb Bud by Electroporation.
Transgene Introduction into the Chick Limb Bud by Electroporation.
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通过电穿孔将转基因引入鸡肢芽。
DOI：
10.1007/978-1-4939-7216-6_13
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发表时间：
2017
期刊：
影响因子：
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通讯作者：
Tanaka M.
中科院分区：
文献类型：
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作者：
Ueda S.;Suzuki T.;Tanaka M.
电穿孔使生物异常分子，如dna、rna、sirna和morpholinos，能够以空间和时间限制的方式传递到鸡胚胎中。电穿孔技术的最新进展使我们能够将转基因传递到肢体芽的受限区域，并分析增强子在肢体场中的功能。本文介绍了电穿孔法在肢体领域引入转基因及其在增强子分析中的应用。
Electroporation enables delivering bionanomolecules, such as DNAs, RNAs, siRNAs, and morpholinos, into chick embryos in a spatially and temporally restricted fashion. Recent advances in electroporation techniques allowed us to deliver transgenes into the restricted area of the limb bud and to analyze the function of the enhancers in the limb field. Here, we describe the introduction of transgenes by electroporation in the limb field and its application on enhancer analysis.