The Effect of Norepinephrine and Dibutyryl Cyclic Adenosine Monophosphate on Cation Transport in Duck Erythrocytes
The Effect of Norepinephrine and Dibutyryl Cyclic Adenosine Monophosphate on Cation Transport in Duck Erythrocytes
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去甲肾上腺素和二丁酰环磷酸腺苷对鸭红细胞阳离子转运的影响
DOI：
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发表时间：
1971
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影响因子：
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通讯作者：
J. Orloff
中科院分区：
文献类型：
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作者：
D. Riddick;F. Kregenow;J. Orloff
新鲜制备的鸭红细胞，在血浆或含去甲肾上腺素的等渗合成培养基中孵育（两种培养基的[K]均<2.5 mM），维持水和电解质组成处于稳态如果在不含去甲肾上腺素的合成培养基中或在含有β-肾上腺素能阻滞剂的血浆中孵育，当加入普萘洛尔（propranolol）时，细胞失去水和电解质（主要是KCl），直到达到新的稳态（较低稳态）。水和电解质从等渗溶液向上稳态水平的再蓄积需要加入去甲肾上腺素和KCl。当培养基中K和去甲肾上腺素的浓度分别为15 mM和10-7 M时，再积累最大。二丁酰环AMP（10-2 M）在较低稳态细胞中模拟去甲肾上腺素。虽然类似的效果在上稳态细胞没有建立与确定性，它建议，在鸭红细胞中的水和电解质运动的儿茶酚胺诱导的净变化是一个膜结合腺苷酸环化酶系统的活性刺激的结果。
Freshly prepared duck erythrocytes, incubated either in plasma or an isotonic synthetic medium containing norepinephrine ([K] of both media ∼ 2.5 mM), maintain water and electrolyte composition in the steady state (upper steady state) for at least 90 min. If incubated in the synthetic medium without norepinephrine or in plasma to which a β-adrenergic blocking agent (propranolol) is added, the cells lose both water and electrolyte (predominantly KCl) until a new steady state is reached (lower steady state). Reaccumulation of water and electrolyte from isotonic solutions toward the upper steady-state levels requires the addition of norepinephrine and KCl. Reaccumulation is maximal when the concentration of K and norepinephrine in the medium is 15 mM and 10-7 M, respectively. Dibutyryl cyclic-AMP (10-2 M) mimics norepinephrine in lower steady-state cells. Although an analogous effect in upper steady-state cells was not established with certainty, it is proposed that the catecholamine-induced net changes in water and electrolyte movement in duck erythrocytes are a consequence of stimulation of the activity of a membrane-bound adenyl cyclase system.