Drug resistance-conferring mutations in Mycobacterium tuberculosis from Madang, Papua New Guinea.
Drug resistance-conferring mutations in Mycobacterium tuberculosis from Madang, Papua New Guinea.
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DOI：
10.1186/1471-2180-12-191
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发表时间：
2012-09-04
期刊：
影响因子：
4.2
通讯作者：
Beck HP
中科院分区：
文献类型：
--
作者：
Ballif M;Harino P;Ley S;Coscolla M;Niemann S;Carter R;Coulter C;Borrell S;Siba P;Phuanukoonnon S;Gagneux S;Beck HP
监测结核分枝杆菌的耐药性对于遏制结核病的传播至关重要。不幸的是，巴布亚新几内亚目前没有药物敏感性测试，这损害了该国的结核病控制。我们首次在巴布亚新几内亚马当报告了与一线和二线抗结核药物耐药相关的结核分枝杆菌突变。进行了分子聚类分析以确定该地区的结核分枝杆菌传播。表型药敏试验显示15.7%的患者对至少一种药物耐药，5.2%的患者耐多药（MDR）。利福平耐药菌株rpoB突变为D516F、D516Y或S531L；异烟肼耐药菌株存在katG S315T或inhA启动子C15T突变；链霉素耐药菌株突变为rpsL K43R、K88Q、K88R)、rrs A514C或gidB V77G。分子聚类分析显示有耐药菌株传播的证据。我们观察到巴布亚新几内亚Madang地区耐多药结核病的大量发病率与特定基因的突变有关。因此，迫切需要密切监测耐药性，特别是在存在耐药结核分枝杆菌传播的情况下。在巴布亚新几内亚缺乏表型药敏试验的情况下，进行耐药性监测的分子分析将是有利的。
Monitoring drug resistance in Mycobacterium tuberculosis is essential to curb the spread of tuberculosis (TB). Unfortunately, drug susceptibility testing is currently not available in Papua New Guinea (PNG) and that impairs TB control in this country. We report for the first time M. tuberculosis mutations associated with resistance to first and second-line anti-TB drugs in Madang, PNG. A molecular cluster analysis was performed to identify M. tuberculosis transmission in that region. Phenotypic drug susceptibility tests showed 15.7% resistance to at least one drug and 5.2% multidrug resistant (MDR) TB. Rifampicin resistant strains had the rpoB mutations D516F, D516Y or S531L; Isoniazid resistant strains had the mutations katG S315T or inhA promoter C15T; Streptomycin resistant strains had the mutations rpsL K43R, K88Q, K88R), rrs A514C or gidB V77G. The molecular cluster analysis indicated evidence for transmission of resistant strain. We observed a substantial rate of MDR-TB in the Madang area of PNG associated with mutations in specific genes. A close monitoring of drug resistance is therefore urgently required, particularly in the presence of drug-resistant M. tuberculosis transmission. In the absence of phenotypic drug susceptibility testing in PNG, molecular assays for drug resistance monitoring would be of advantage.
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影响因子：
9.4
作者：
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