Multiplex PCR for the detection of tetracycline resistant genes
Multiplex PCR for the detection of tetracycline resistant genes
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DOI：
10.1006/mcpr.2001.0363
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发表时间：
2001-08-01
影响因子：
3.3
通讯作者：
Mulvey, M
中科院分区：
文献类型：
--
作者：
Ng, LK;Martin, I;Mulvey, M
选择特异性引物对用于PCR扩增革兰氏阳性和阴性微生物中常见的14个四环素耐药基因。在多重PCR反应中使用引物对的组合以检测如下的tel基因的特定组;组I：泰特（B）、泰特（C）、泰特（D）;组II：泰特（A）、tet（E）、tet（G）;组III：tet（A）、泰特（E）、tet（G）。泰特（K）、泰特（L）、泰特（M）、泰特（O）、泰特（S）;第IV组：tetA（P）、泰特（Q）、泰特（X）。为了测试：多重PCR，I组和II组用于25个临床分离的沙门氏菌血清型鼠伤寒沙门氏菌DT 104。用Ⅲ组引物对19株耐甲氧西林金黄色葡萄球菌进行了检测。当与使用单个PCR靶向每个基因相比时，多重PCR在分析大量菌株的劳动力和成本方面应导致显著节省。它也可能是一个有用的方法来区分四环素耐药的类型时，作为一个额外的标志物，用于暴发调查和监测的目的。
Specific primer pairs were selected for the PCR amplification of 14 tetracycline resistant genes commonly found in Gram positive and Cram negative organisms. Combinations of primer pairs were used in multiplex PCR reactions to detect specific groups of tel genes as follows; Group I: tet(B), tet(C), tet(D); Group II: tet(A), tet(E), tet(G); Group III. tet(K), tet(L), tet(M), tet(O), tet(S); Group IV: tetA(P), tet(Q), tet(X). To test: the multiplex PCR, Groups I and II were used on 25 clinical isolates of Salmonella enterica serovar Typhimurium DT104. Group III primers were used to investigate 19 clinical isolates of methicillin-resistant Staphylococcus aureus. Multiplex PCR should result in significant savings in terms of labour and cost in analysis of a large number of strains when compared with using an individual PCR for targeting each gene. It may also be a useful method to differentiate the types of tetracycline resistance when used as an additional marker for the purpose of outbreak investigation and surveillance.