Huh-7 or HepG2 cells: which is the better model for studying human apolipoprotein-B100 assembly and secretion?
Huh-7 or HepG2 cells: which is the better model for studying human apolipoprotein-B100 assembly and secretion?
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DOI：
10.1194/jlr.d008888
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发表时间：
2011-01-01
影响因子：
6.5
通讯作者：
Fisher, Edward A.
中科院分区：
文献类型：
--
作者：
Meex, Steven J. R.;Andreo, Ursula;Fisher, Edward A.
载脂蛋白-B100(ApoB100)是肝脏组装和分泌极低密度脂蛋白(VLDL)所必需的蛋白质。肝癌细胞系HepG2已成为研究人载脂蛋白B-100合成和分泌的首选细胞系。尽管通常使用HepG2细胞来研究apoB100的代谢，但与体内分泌的极低密度脂蛋白相比，它们分泌的颗粒相对致密，缺乏脂质。最近，Huh-7细胞被用作HepG2细胞的替代模型，其隐含的假设是Huh-7细胞在脂蛋白代谢的某些方面具有优势，包括VLDL的分泌。在这项研究中，我们提出了一种假设，即Huh-7细胞分泌的apoB100脂蛋白颗粒的光谱更接近于人类肝脏的自然状态。我们发现Huh-7细胞在外源性脂质、微粒体甘油三酯转移蛋白(MTP)抑制和蛋白酶体抑制apoB100的分泌、恢复和降解方面与HepG2细胞相似。然而，与HepG2细胞相比，抑制MEK-ERK并不能纠正VLDL分泌的缺陷。作为人类载脂蛋白B100-脂蛋白代谢的一般模型，HH-7细胞似乎没有提供任何优于HepG2细胞的优势。-MEEX，S.J.R.，U·Andreo，J.D.斯帕克斯和E.A.Fisher。HH-7或HepG2细胞：研究人载脂蛋白-B100的组装和分泌，哪个模型更好？J.Lipid Res.2011年。52：152-158。
Apolipoprotein-B100 (apoB100) is the essential protein for the assembly and secretion of very low density lipoproteins (VLDL) from liver. The hepatoma HepG2 cell line has been the cell line of choice for the study of synthesis and secretion of human apoB-100. Despite the general use of HepG2 cells to study apoB100 metabolism, they secrete relatively dense, lipid-poor particles compared with VLDL secreted in vivo. Recently, Huh-7 cells were adopted as an alternative model to HepG2 cells, with the implicit assumption that Huh-7 cells were superior in some respects of lipoprotein metabolism, including VLDL secretion. In this study we addressed the hypothesis that the spectrum of apoB100 lipoprotein particles secreted by Huh-7 cells more closely resembles the native state in human liver. We find that Huh-7 cells resemble HepG2 cells in the effects of exogenous lipids, microsomal triglyceride transfer protein (MTP)-inhibition, and proteasome inhibitors of apoB100 secretion, recovery, and degradation. In contrast to HepG2 cells, however, MEK-ERK inhibition does not correct the defect in VLDL secretion. Huh-7 cells do not appear to offer any advantages over HepG2 cells as a general model of human apoB100-lipoprotein metabolism.-Meex, S. J. R., U. Andreo, J. D. Sparks, and E. A. Fisher. Huh-7 or HepG2 cells: which is the better model for studying human apolipoprotein-B100 assembly and secretion? J. Lipid Res. 2011. 52: 152-158.