Improvement of cell attachment capabilities of poly-l-lactic acid films by modification of surface properties with ion-beam irradiation
Improvement of cell attachment capabilities of poly-l-lactic acid films by modification of surface properties with ion-beam irradiation
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DOI：
10.1016/j.surfcoat.2012.12.047
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发表时间：
2013-03
影响因子：
5.4
通讯作者：
Toshiyuki Tanaka;Yoshiaki Suzuki;K. Tsuchiya;H. Yajima
中科院分区：
文献类型：
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作者：
Toshiyuki Tanaka;Yoshiaki Suzuki;K. Tsuchiya;H. Yajima
在这项研究中，研究了表面特性（包括离子束照射的聚（L-乳酸）薄膜的碳键状态）与细胞附着能力之间的相关性。使用H+、N2+或Kr+束以50keV的能量照射薄膜表面，注量范围为1×1013至1×1015ions/cm2。通过X射线光电子能谱（XPS）、拉曼光谱和水的接触角测量来评估化学成分、碳结构和辐照表面的润湿性。碳化随着离子质量和/或辐照通量进行，随后与未辐照表面相比，辐照表面变得疏水。将小鼠成纤维细胞 (L929) 接种到未辐射和辐射表面上以评估细胞附着能力。这些结果表明，适量的CC基团适合细胞附着和增殖，而过量则具有抑制作用。
In this study, the correlation between surface properties, including the carbon-bonding state for ion-beam irradiated poly(l-lactic acid) films, and cell attachment capabilities was investigated. H+, N2+, or Kr+beams were used to irradiate the film-surface at an energy of 50keV with fluences ranging from 1×1013to 1×1015ions/cm2. Chemical compositions, carbon structure, and wettability of the irradiated surfaces were evaluated by X-ray photoelectron spectroscopy (XPS), Raman spectroscopy, and contact angle measurement of water. Carbonization proceeded with ion mass and/or irradiation fluence, and subsequently the irradiated surfaces became hydrophobic compared to the non-irradiated surface. Mouse fibroblasts (L929) were seeded onto the non-irradiated and irradiated surfaces to evaluate cell attachment capabilities. These results indicate that a moderate amount of CC groups is suitable for cell attachment and proliferation, and that an excess is inhibitory.