Decoding cilia function: Defining specialized genes required for compartmentalized cilia biogenesis
Decoding cilia function: Defining specialized genes required for compartmentalized cilia biogenesis
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DOI：
10.1016/s0092-8674(04)00412-x
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发表时间：
2004-05-14
期刊：
影响因子：
64.5
通讯作者：
Zuker, CS
中科院分区：
文献类型：
--
作者：
Avidor-Reiss, T;Maer, AM;Zuker, CS
祖先真核生物鞭毛的进化是细胞器的一个例子，它在一些现代真核生物中变得可有可无，而在另一些真核生物中仍然是必不可少的运动和感觉器官。为了帮助定义纤毛形成和功能所需的特殊蛋白质的全部成分，我们使用比较基因组学来分析具有原型纤毛、修饰纤毛或无纤毛的生物体的基因组，并鉴定了大约 200 个在非纤毛真核生物基因组中不存在但在纤毛生物体中保守的基因。重要的是，超过 80% 的已知参与纤毛功能的祖先蛋白质都包含在这个小集合中。然后，我们使用果蝇作为模型系统，描述了纤毛发生所必需的一个新的蛋白质家族（OSEG：外节）。我们发现 osegs 编码纤毛区室特有的专门转运途径的成分，并且与典型的细胞内转运蛋白相关。
The evolution of the ancestral eukaryotic flagellum is an example of a cellular organelle that became dispensable in some modern eukaryotes while remaining an essential motile and sensory apparatus in others. To help define the repertoire of specialized proteins needed for the formation and function of cilia, we used comparative genomics to analyze the genomes of organisms with prototypical cilia, modified cilia, or no cilia and identified similar to200 genes that are absent in the genomes of nonciliated eukaryotes but are conserved in ciliated organisms. Importantly, over 80% of the known ancestral proteins involved in cilia function are included in this small collection. Using Drosophila as a model system, we then characterized a novel family of proteins (OSEGs: outer segment) essential for ciliogenesis. We show that osegs encode components of a specialized transport pathway unique to the cilia compartment and are related to prototypical intracellular transport proteins.