Differentially expressed forms of 1-L-myo-inositol-1-phosphate synthase (EC 5.5.1.4) in Phaseolus vulgaris
Differentially expressed forms of 1-L-myo-inositol-1-phosphate synthase (EC 5.5.1.4) in Phaseolus vulgaris
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DOI：
10.1074/jbc.271.29.17215
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发表时间：
1996-07-19
影响因子：
4.8
通讯作者：
Wang, XH
中科院分区：
文献类型：
--
作者：
Johnson, MD;Wang, XH
我们鉴定了两种具有 1-L-肌醇-1-磷酸合酶 (MI-1-P 合酶) 活性的不同多肽，它们在菜豆发育过程中差异表达。胚胎和胚胎后发育过程中 MI-1-P 合酶的 Western 分析、酶测定和部分纯化表明，其表达在时间和空间上受到调节。可溶性蛋白质的发育 Western 分析检测到约 33 kDa 的小蛋白质，在胚胎发生的球状阶段（第二阶段）和成熟根中具有 MI-1-P 合酶活性。这种小蛋白的表达也在分级叶绿体的类囊体膜中富集，尽管对总可溶性叶蛋白的西方分析显示没有交叉反应物质。相反，在幼苗的绿色子叶和幼根的胚胎发生的子叶期（第四阶段）中存在具有 MI-1-P 合酶活性的较大蛋白，约 56 kDa。
We have characterized two distinct polypeptides with 1-L-myo-inositol-1-phosphate synthase (MI-1-P synthase) activity that are differentially expressed during development in Phaseolus vulgaris. Western analyses, enzyme assays, and partial purification of MI-1-P synthase during embryonic and postembryonic development show that its expression is temporally and spatially regulated.Developmental Western analyses of soluble proteins detect a small protein, approximately 33 kDa, with MI-1-P synthase activity during the globular stage (stage II) of embryogenesis and in mature roots. Expression of this small protein is also enriched in thylakoidal membranes of fractionated leaf chloroplasts, although Western analyses of total soluble leaf proteins show no cross-reacting material, In contrast, a larger protein, approximately 56 kDa, with MI-1-P synthase activity is present during the cotyledonary phase (stage IV) of embryogenesis in green cotyledons of seedlings and in young roots.