Relationship between p21 and p53 expression, human papilloma virus infection and malignant transformation in sinonasal-inverted papilloma
Relationship between p21 and p53 expression, human papilloma virus infection and malignant transformation in sinonasal-inverted papilloma
复制标题
DOI：
10.1016/j.clon.2005.11.001
复制
发表时间：
2006-05-01
影响因子：
3.4
通讯作者：
Tsukuda, M
中科院分区：
文献类型：
--
作者：
Katori, H;Nozawa, A;Tsukuda, M
目的：为探讨鼻腔鼻窦内翻性乳头状瘤（sinonasalinvertebralpapilloma-inverted）中p21和p53蛋白表达与人乳头状瘤病毒（HPV）感染及恶性转化的关系，材料和方法：采用单克隆抗体p21和p53对鼻腔鼻窦内翻性乳头状瘤、内翻性乳头状瘤伴异型增生、内翻性乳头状瘤伴癌及浸润性鳞状细胞癌（invertebralpapilloma-invertebralcarcinoma，SCC）进行染色。结果：与正常鼻粘膜相比，伴重度不典型增生的IP、伴癌的IP和浸润性癌的IP中p21和p53蛋白表达明显增强。与HPV 6/11和16/18阴性组相比，在HPV 6/11和16/18阳性组中观察到IP中异型增生显著增加。在HPV 16/18阳性IP中观察到p21和p53的表达显著降低，与HPV 16/18阴性IP.Conclusions相比：我们的数据提高了检测p21，p53和HPV可能有助于筛选出具有异型增生或癌潜力的乳头状瘤病变的可能性。
Aims: To identify the relationship between p21 and p53 expression, human papilloma virus (HPV) infection and malignant transformation in sinonasal-inverted papilloma.Material and methods: Nasal tissues, exophytic papilloma, inverted papilloma (IP) with dysplasia, IP with carcinoma and invasive squamous cell carcinoma (SCC) were stained with the monoclonal antibodies p21 and p53. In-situ hybridisation for HPV DNA was also carried out for types 6/11, 16/18 and 31/33.Results: Significant increased staining of p21 and p53 was observed in IP with severe dysplasia, IP with carcinoma and invasive carcinoma compared with control nasal mucosa. A significant increase of dysplasia was observed in IP in the HPV 6/11 and 16/18-positive group, compared with the HPV 6/11 and 16/18-negative group. Significant decrease in expression of p21 and p53 was observed in HPV 16/18-positive IP compared with HPV 16/18-negative IP.Conclusions: Our data raise the possibility that testing for p21, p53 and HPV may help to screen out papilloma lesions with a potential for dysplasia or carcinoma.