A combination of genome-wide association study and transcriptome analysis in leaf epidermis identifies candidate genes involved in cuticular wax biosynthesis in Brassica napus.
A combination of genome-wide association study and transcriptome analysis in leaf epidermis identifies candidate genes involved in cuticular wax biosynthesis in Brassica napus.
复制标题
叶表皮全基因组关联研究和转录组分析相结合，鉴定出参与甘蓝型油菜角质层蜡生物合成的候选基因
DOI：
10.1186/s12870-020-02675-y
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发表时间：
2020-10-06
影响因子：
5.3
通讯作者：
Ni Y
中科院分区：
文献类型：
--
作者：
Jin S;Zhang S;Liu Y;Jiang Y;Wang Y;Li J;Ni Y
BackgroundBrassica napusL。是世界上最重要的油料作物之一。然而，气候变化引起的环境胁迫对其产量和品质产生了负面影响。众所周知，角质层蜡可以保护植物免受各种非生物/生物胁迫。解剖表皮蜡质背后的遗传和生化基础，对培育抗逆性强的品种具有重要意义。结果对192B进行了全基因组关联研究。利用油菜品种和自交系鉴定了与叶蜡相关的单核苷酸多态性（snp）。共发现202个snp与31个蜡质性状（包括蜡质总覆盖度、蜡类和蜡质化合物数量）显著相关。接下来，从高蜡质负荷（HW）和低蜡质负荷（LW）的叶片中分离表皮皮，并用于分析gwas鉴定的所有基因的转录谱。结果显示，147个SNP在HW和LW之间存在差异表达，其中344个SNP对应基因在LW中表达上调，而448个SNP对应基因在LW中表达下调。根据基因注释信息，将部分差异表达基因分类为植物酰基脂质代谢途径，包括脂肪酸相关途径、蜡质与角质层生物合成途径和蜡质分泌途径。一些参与细胞壁形成和应激反应的基因也已被确定。结论GWAS与转录组学分析相结合，揭示了许多与蜡相关的直接或间接基因及其相关的snp。这些结果可以为进一步验证标记辅助育种的snp提供线索，并为蜡生物合成的遗传控制和提高b的抗逆性提供新的见解。显著。
BackgroundBrassica napusL. is one of the most important oil crops in the world. However, climate-change-induced environmental stresses negatively impact on its yield and quality. Cuticular waxes are known to protect plants from various abiotic/biotic stresses. Dissecting the genetic and biochemical basis underlying cuticular waxes is important to breed cultivars with improved stress tolerance.ResultsHere a genome-wide association study (GWAS) of 192B. napuscultivars and inbred lines was used to identify single-nucleotide polymorphisms (SNPs) associated with leaf waxes. A total of 202 SNPs was found to be significantly associated with 31 wax traits including total wax coverage and the amounts of wax classes and wax compounds. Next, epidermal peels from leaves of both high-wax load (HW) and low-wax load (LW) lines were isolated and used to analyze transcript profiles of all GWAS-identified genes. Consequently, 147 SNPs were revealed to have differential expressions between HW and LW lines, among which 344 SNP corresponding genes exhibited up-regulated while 448 exhibited down-regulated expressions in LW when compared to those in HW. According to the gene annotation information, some differentially expressed genes were classified into plant acyl lipid metabolism, including fatty acid-related pathways, wax and cutin biosynthesis pathway and wax secretion. Some genes involved in cell wall formation and stress responses have also been identified.ConclusionsCombination of GWAS with transcriptomic analysis revealed a number of directly or indirectly wax-related genes and their associated SNPs. These results could provide clues for further validation of SNPs for marker-assisted breeding and provide new insights into the genetic control of wax biosynthesis and improving stress tolerance ofB. napus.