B Cells Produce Type 1 IFNs in Response to the TLR9 Agonist CpG-A Conjugated to Cationic Lipids.
B Cells Produce Type 1 IFNs in Response to the TLR9 Agonist CpG-A Conjugated to Cationic Lipids.
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DOI：
10.4049/jimmunol.1700348
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发表时间：
2017-08-01
期刊：
影响因子：
--
通讯作者：
Pierce SK
中科院分区：
文献类型：
--
作者：
Akkaya M;Akkaya B;Miozzo P;Rawat M;Pena M;Sheehan PW;Kim AS;Kamenyeva O;Kabat J;Bolland S;Chaturvedi A;Pierce SK
B cells express the innate receptor, toll-like receptor 9 (TLR9), which signals in response to unmethylated CpG sequences in microbial DNA. Of the two major classes of CpG-containing oligonucleotides, CpG-A appears restricted to inducing type 1 interferon (type 1 IFN) in innate immune cells and CpG-B to activating B cells to proliferate and produce antibodies and inflammatory cytokines. Although CpGs are candidates for adjuvants to boost innate and adaptive immunity, our understanding of the effect of CpG-A and CpG-B on B cell responses is incomplete. Here we show that both CpG-B and CpG-A activated B cells in vitro to proliferate, secrete antibodies and IL-6 and that neither CpG-B nor CpG-A alone induced type 1 IFN production. However, when incorporated into the cationic lipid, DOTAP, CpG-A, but not CpG-B induced a type 1 IFN response in B cells in vitro and in vivo. We provide evidence that differences in the function of CpG-A and CpG-B may be related to their intracellular trafficking in B cells. These findings fill an important gap in our understanding of the B cell response to CpGs with implications for the use of CpG-A and CpG-B as immunomodulators.
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影响因子：
6.4
作者：
Gordon EB;Hart GT;Tran TM;Waisberg M;Akkaya M;Skinner J;Zinöcker S;Pena M;Yazew T;Qi CF;Miller LH;Pierce SK
通讯作者：
Pierce SK
DOI：
10.1084/jem.20031237
发表时间：
2004-01-05
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
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通讯作者：
Cheng G
影响因子：
64.8
作者：
Honda, K;Ohba, Y;Taniguchi, T
通讯作者：
Taniguchi, T
DOI：
10.1126/science.1187029
发表时间：
2010-09-17
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Sasai M;Linehan MM;Iwasaki A
通讯作者：
Iwasaki A
DOI：
10.1084/jem.20111675
发表时间：
2012-05-07
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Barr TA;Shen P;Brown S;Lampropoulou V;Roch T;Lawrie S;Fan B;O'Connor RA;Anderton SM;Bar-Or A;Fillatreau S;Gray D
通讯作者：
Gray D