Metal complexes as optical probes for DNA sensing and imaging.
Metal complexes as optical probes for DNA sensing and imaging.
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DOI：
10.1016/j.cbpa.2021.02.007
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发表时间：
2021-03
影响因子：
7.8
通讯作者：
Jessica Berrones Reyes;M. Kuimova;R. Vilar
中科院分区：
文献类型：
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作者：
Jessica Berrones Reyes;M. Kuimova;R. Vilar
过渡金属和镧系金属配合物具有丰富的物理化学性质，可用于细胞成像、生物传感和光疗。这种发光化合物的应用之一是核酸的检测和可视化。本文综述了近年来发光金属配合物（包括Re I、Ru II、OsII、Ir III、Pt II、Eu III和Tb III）作为DNA光学探针的研究进展，包括选择性结合非双链体DNA拓扑结构（如四链体、i-基序和DNA错配）的化合物。我们讨论了基于金属的发光配合物在细胞成像中的应用，包括时间分辨显微镜和超分辨率技术。它们在生物传感和光疗中的应用在相关章节中简要提及。
Transition and lanthanide metal complexes have rich photophysical properties that can be used for cellular imaging, biosensing and phototherapy. One of the applications of such luminescent compounds is the detection and visualisation of nucleic acids. In this brief review, we survey the recent literature on the use of luminescent metal complexes (including ReI, RuII, OsII, IrIII, PtII, EuIIIand TbIII) as DNA optical probes, including examples of compounds that bind selectively to non-duplex DNA topologies such as quadruplex,i-motif and DNA mismatches. We discuss the applications of metal-based luminescent complexes in cellular imaging, including time-resolved microscopy and super-resolution techniques. Their applications in biosensing and phototherapy are briefly mentioned in the relevant sections.